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MEC-17 es una alfa-tubulina acetiltransferasa
Jyothi S Akella1, Dorota Wloga, Jihyun Kim
1Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA.
Nature
|September 11, 2010
Resumen
Los investigadores identificaron a MEC-17 como la enzima responsable de acetilizar la alfa-tubulina en K40, una modificación post-traducional clave en los microtúbulos. Este descubrimiento aclara el mecanismo detrás de la función y la estabilidad de los microtúbulos en varios organismos.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Los microtúbulos son estructuras celulares cruciales cuyas funciones están reguladas por modificaciones post-traducionales (PTM) de las subunidades de tubulina.
- La acetilación de la alfa-tubulina K40 es un PTM conservado en el lado luminal de los microtúbulos, pero la enzima responsable seguía siendo desconocida.
- Comprender la acetilación de microtúbulos es vital para comprender los procesos celulares y los posibles mecanismos de enfermedad.
Objetivo del estudio:
- Para identificar la alfa-tubulina acetiltransferasa responsable de la acetilación del K40.
- Investigar la importancia funcional de MEC-17 en la acetilación de microtúbulos y en los procesos celulares.
- Para aclarar el papel de MEC-17 en diferentes organismos modelo.
Principales métodos:
- Ensayos bioquímicos para probar la actividad de la acetiltransferasa de MEC-17 in vitro.
- Experimentos de alteración genética y eliminación en Tetrahymena, pez cebra y Caenorhabditis elegans.
- Análisis fenotípico de organismos mutantes y empobrecidos para evaluar la función de los microtúbulos y los defectos celulares.
Principales resultados:
- MEC-17 fue identificado como una alfa-tubulina acetiltransferasa específica de K40.
- La interrupción de MEC-17 condujo a microtúbulos más labiles y a mutaciones fenocopiadas de K40R alfa-tubulina.
- El agotamiento de MEC-17 en el pez cebra causó defectos neuromusculares, y en C. elegans, se requería de forma redundante para la función neuronal del receptor táctil a través de la acetilación de MEC-12.
Conclusiones:
- MEC-17 es la enzima responsable de la única PTM luminal de la acetilación de la alfa-tubulina K40.
- MEC-17 juega un papel crítico en la estabilidad de los microtúbulos y la función neuronal en diferentes especies.
- Este hallazgo proporciona una visión clave de la regulación de la función de los microtúbulos a través de PTMs específicos.
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