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La señalización de la auxina basada en la superficie celular y la rho GTPasa controla la interdigitación celular en
Tongda Xu1, Mingzhang Wen, Shingo Nagawa
1Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA.
Cell
|October 5, 2010
Resumen
Este estudio revela una vía de señalización de la auxina citoplasmática que controla la forma de las células vegetales. La auxina activa las Rho GTPasas para regular el crecimiento interdigitado de las células del pavimento de las hojas de Arabidopsis.
Área de la Ciencia:
- Biología Vegetal Biología Vegetal
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La auxina es una hormona vegetal crucial que regula el desarrollo y la formación de patrones.
- La señalización de la auxina nuclear se entiende, pero las funciones citoplasmáticas, como la polarización celular, siguen siendo en gran medida inexploradas.
Objetivo del estudio:
- Para investigar los mecanismos inexplorados de señalización de la auxina en el citoplasma.
- Para dilucidar cómo la auxina coordina la expansión celular y la polarización en las células epidérmicas de las plantas.
Principales métodos:
- Investigó la biosíntesis de auxinas y las mutaciones de exportación en Arabidopsis.
- Se analizó el papel de las Rho GTPasas (ROP2 y ROP6) en la interdigitación de células de pavimento.
- Examinó la participación de la proteína 1 en la señalización de la auxina.
Principales resultados:
- La señalización de la auxina citoplasmática modula el crecimiento interdigitado de las células epidermales del pavimento de la hoja de Arabidopsis.
- La alteración de la biosíntesis de auxina o la exportación de PINFORMED 1 interrumpe la interdigitación de las células del pavimento.
- La auxina activa rápidamente las GTPasas Rho ROP2 y ROP6, promoviendo el lóbulo complementario y la formación de hendiduras.
- Esta activación es dependiente de la proteína AUXIN 1.
Conclusiones:
- Una nueva vía de señalización de la auxina citoplasmática basada en la Rho GTPasa regula la forma de las células vegetales.
- Este mecanismo es crítico para la coordinación espacial de la expansión celular en las células del pavimento.
- Los hallazgos revelan nuevos conocimientos sobre el papel de la auxina más allá de la expresión génica nuclear.
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