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Updated: Jun 8, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
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Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

Un oxidante caliente, el radical 3-NO2Y122, desenmascara las compuertas conformacionales en la ribonucleótido

Kenichi Yokoyama1, Ulla Uhlin, JoAnne Stubbe

  • 1Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, United States.

Journal of the American Chemical Society
|October 9, 2010
PubMed
Resumen

Este estudio revela cómo una ribonucleótido reductasa (RNR) modificada de Escherichia coli desacopla la transferencia de electrones acoplados a protones (PCET) mediante el uso de un radical de nitrotirosilo (NO(2) Y(•)). Este desacoplamiento desenmascara el paso de entrada conformacional que limita la velocidad de la enzima, proporcionando nuevos conocimientos sobre la catálisis RNR.

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