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Se requieren subunidades del complejo de unión de exones para empalmar la Drosophila MAP quinasa, un gran gen
Jean-Yves Roignant1, Jessica E Treisman
1Kimmel Center for Biology and Medicine of the Skirball Institute, NYU School of Medicine, Department of Cell Biology, 540 First Avenue, New York, NY 10016, USA.
Cell
|October 16, 2010
Resumen
La subunidad del complejo de unión de exones (EJC) Mago nashi (mago) es crucial para el empalme del gen MAPK. La pérdida de mago interrumpe el desarrollo de los fotorreceptores al afectar la señalización del receptor del factor de crecimiento epidérmico.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- El complejo de unión de exones (EJC) es un complejo de proteínas ensamblado en ARNm empalmados.
- El EJC juega un papel en la traducción, localización y degradación del ARNm.
- Las mutaciones en los componentes del EJC pueden conducir a defectos del desarrollo.
Objetivo del estudio:
- Para investigar la función de Drosophila mago nashi (mago), una subunidad del núcleo EJC.
- Comprender el papel del mago en la diferenciación de los fotorreceptores y la expresión génica.
Principales métodos:
- Aislamiento de mutaciones mago en Drosophila.
- Análisis de los niveles de ARNm de MAPK y los patrones de empalme.
- Evaluación de la señalización del receptor del factor de crecimiento epidérmico.
- Investigación del efecto de mago en la expresión génica heterocromática en células cultivadas.
Principales resultados:
- La pérdida de Mago afecta específicamente a la diferenciación de los fotorreceptores.
- El agotamiento de Mago reduce los niveles de ARNm MAPK y perjudica la señalización del receptor del factor de crecimiento epidérmico.
- Mago se requiere para el empalme MAPK adecuado, no la transcripción o la estabilidad.
- Mago knockdown afecta de manera desproporcionada a los genes grandes en la heterocromatina.
Conclusiones:
- Mago juega un papel nuclear crítico en el empalme de intrones específicos.
- El EJC, a través de mago, regula la expresión de MAPK, esencial para el desarrollo del ojo.
- La función de Mago se extiende a la regulación de los genes en la heterocromatina.
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