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Detección a temperatura ambiente de la absorción de una sola molécula por contraste fototérmico
A Gaiduk1, M Yorulmaz, P V Ruijgrok
1Institute of Physics, Leiden University, Post Office Box 9504, 2300 RA Leiden, Netherlands.
Science (New York, N.Y.)
|October 16, 2010
Resumen
Desarrollamos una técnica de imagen fototérmica para visualizar moléculas individuales de colorante azo no fluorescente. Este método ofrece un alto contraste y un foto blanqueamiento mínimo, superando las limitaciones de las imágenes de fluorescencia tradicionales.
Área de la Ciencia:
- Óptica y Fotónica.
- La espectroscopia es una técnica de espectroscopia.
- Ciencia de los materiales Ciencia de los materiales.
Sus antecedentes:
- Las imágenes de una sola molécula tradicionalmente se basan en la detección de fluorescencia.
- Los métodos de fluorescencia pueden ser limitados por el foto blanqueamiento y la dispersión de fondo.
- Las moléculas no fluorescentes son difíciles de visualizar a nivel de una sola molécula.
Objetivo del estudio:
- Desarrollar un nuevo método para obtener imágenes de moléculas no fluorescentes individuales.
- Para utilizar efectos fototérmicos para la detección molecular.
- Para evaluar el rendimiento y las limitaciones de esta nueva técnica.
Principales métodos:
- Imágenes de una sola molécula de moléculas de colorante azoico en el glicerol.
- Utilizando el efecto fototérmico generado por la iluminación intensa.
- Medición del efecto refractivo del calor liberado para contraste.
- Investigando el foto blanqueamiento y la relación señal-ruido.
Principales resultados:
- Se logró una relación señal-ruido de ~10 para moléculas individuales con integración de 300 ms.
- Se ha demostrado un foto blanqueamiento mínimo en ausencia de oxígeno (más de 10 minutos).
- Se observó un rápido foto blanqueamiento en soluciones oxigenadas (promedio ~ 1 minuto).
- No se observó parpadeo en la señal de absorción.
Conclusiones:
- La imagen fototérmica es una técnica viable para moléculas no fluorescentes individuales.
- El método proporciona contraste basado en la absorción, independientemente de la dispersión.
- El foto blanqueamiento se reduce significativamente en ambientes desoxigenados.
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