Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Cross-reactivity00:42

Cross-reactivity

Overview
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Preparation of water-soluble sodium polyglucuronates from water-insoluble glucans by NaBr-free TEMPO-catalyzed oxidation with sodium dichloroisocyanurate in water.

Carbohydrate polymers·2026
Same author

NaBr-free TEMPO-catalyzed oxidation of cellulose with sodium dichloroisocyanurate in water at pH 9.

Carbohydrate polymers·2026
Same author

<i>In vitro</i> Evaluation of the Digestive Tolerance of Water-Soluble Sodium β-(1→4)-Polyglucuronate.

Journal of applied glycoscience·2026
Same author

Chemical structures and molar masses of water-soluble TEMPO-oxidized products prepared from 20 % NaOH-treated cellulose.

Carbohydrate polymers·2025
Same author

Can high-molar-mass cellulose molecules be extracted from phosphorylated pulp and TEMPO-oxidized pulps or their nanofibrils using LiCl/DMAc?

Carbohydrate polymers·2025
Same author

Exploring the Allelic Diversity of the Self-Incompatibility Gene Across Natural Populations in Petunia (Solanaceae).

Genome biology and evolution·2024
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Jun 7, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Sistema colaborativo de no auto reconocimiento en autoincompatibilidad basado en S-RNase.

Ken-ichi Kubo1, Tetsuyuki Entani, Akie Takara

  • 1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma 630-0192, Japan.

Science (New York, N.Y.)
|November 6, 2010
PubMed
Resumen
Este resumen es generado por máquina.

Las plantas con flores utilizan la autoincompatibilidad para garantizar la diversidad genética. En Petunia, múltiples proteínas del S-locus F-box (SLF) colaboran para reconocer diversas S-ribonucleasas no autónomas, lo que permite una polinización compatible.

Más Videos Relacionados

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Videos de Experimentos Relacionados

Last Updated: Jun 7, 2026

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses
07:12

Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses

Published on: June 30, 2023

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
08:08

Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses

Published on: June 16, 2020

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Área de la Ciencia:

  • Biología reproductiva de las plantas Biología reproductiva de las plantas
  • Genética molecular genética molecular.
  • Biología evolutiva Biología evolutiva.

Sus antecedentes:

  • La autoincompatibilidad (SI) es un mecanismo crucial en las plantas con flores, que evita la endogamia y promueve la diversidad genética a través del cruce externo.
  • En la familia Solanaceae, el gen S-locus F-box (SLF) codifica el determinante del polen, mientras que las S-ribonucleasas (S-RNasas) representan el determinante del pistilo en SI.
  • Una pregunta clave en la investigación SI es cómo el determinante del polen (SLF) reconoce una amplia gama de determinantes de pistilos no propios (S-RNasas) dada la menor diversidad alélica de SLF.

Objetivo del estudio:

  • Investigar el mecanismo por el cual las proteínas SLF reconocen diversas S-RNasas en Petunia.
  • Para dilucidar la naturaleza colaborativa del determinante del polen en el reconocimiento de las S-RNasas no-auto.
  • Comprender las bases moleculares de la autoincompatibilidad en las solanáceas.

Principales métodos:

  • Se emplearon ensayos funcionales in vivo para probar la actividad de las proteínas SLF en tubos de polen.
  • Se realizaron ensayos de interacción proteica para determinar las especificidades de unión entre las proteínas SLF y S-RNase.
  • Se realizó un análisis comparativo de la secuencia de alelos SLF y S-RNase.

Principales resultados:

  • En Petunia, al menos tres tipos distintos de proteínas SLF divergentes fueron identificados como determinantes funcionales del polen.
  • Cada tipo de proteína SLF demostró la capacidad de reconocer un subconjunto específico de S-RNasas no autónomas.
  • Estos hallazgos sugieren un sistema de reconocimiento colaborativo en lugar de una interacción uno a uno entre SLF y S-RNase.

Conclusiones:

  • El estudio revela un sistema colaborativo de no auto reconocimiento mediado por múltiples proteínas SLF en la autoincompatibilidad de Petunia.
  • Este mecanismo de colaboración permite el reconocimiento de un amplio espectro de S-RNases no autónomas, resolviendo el rompecabezas anterior de la capacidad de reconocimiento de SLF.
  • Los hallazgos contribuyen a una comprensión más profunda de los mecanismos moleculares subyacentes a la autoincompatibilidad de las plantas y al mantenimiento de la diversidad genética.