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Diferentes vías median la inducibilidad del virus de los genes humanos IFN-alfa 1 e IFN-beta
N J MacDonald1, D Kuhl, D Maguire
1Institut für Molekularbiologie I, Universität Zürich, Switzerland.
Cell
|March 9, 1990
Resumen
Las secuencias GAAANN multimerizadas regulan los promotores inducibles por el virus. Diferentes tipos de estas secuencias median respuestas distintas a los interferones (IFN) e IRF-1, que afectan la transcripción génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Genética La genética.
Sus antecedentes:
- Los promotores inducibles por virus a menudo contienen secuencias repetitivas como GAAANN.
- Comprender estos elementos reguladores es crucial para descifrar las interacciones huésped-patógeno.
Objetivo del estudio:
- Diferenciar las funciones funcionales de varios tipos de secuencias (GAAANN) 4 en la inducibilidad viral.
- Para identificar factores específicos de transcripción asociados con estas secuencias.
Principales métodos:
- Análisis de las variantes de secuencia (GAAANN) 4 y sus efectos en la actividad del promotor.
- Ensayos de desplazamiento de movilidad electroforética (EMSA) para identificar la unión a las proteínas.
Principales resultados:
- Se identificaron tres tipos de secuencias (GAAANN) 4, que difieren en su respuesta a los interferones (IFN) e IRF-1.
- Las secuencias de tipo I median la respuesta IFN/IRF-1 y el silenciamiento de genes.
- Las secuencias de tipo II y tipo III exhiben patrones de transcripción distintos y se unen a factores específicos (proteína TG e IEFga, respectivamente).
- Los promotores de IFN-beta e IFN-alfa 1 utilizan diferentes combinaciones de estos elementos, incluida la NF-kappa B.
Conclusiones:
- La multimerización de la secuencia (GAAANN) 4 genera elementos reguladores distintos para la respuesta viral.
- Los elementos tipo I, tipo II y NF-kappa B representan vías separadas que convergen en la inducción viral.
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