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El silenciamiento epigenético mediado por vernalización por un largo ARN intrónico no codificante
1Section of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, the University of Texas at Austin, TX 78712, USA.
Science (New York, N.Y.)
|December 4, 2010
Resumen
La vernalización utiliza el frío para silenciar epigenéticamente el gen FLOWERING LOCUS C (FLC). Un nuevo ARN no codificante intrónico largo, COLDAIR, es esencial para esta represión epigenética inducida por el frío al dirigir PRC2 a FLC.
Área de la Ciencia:
- Biología vegetal Biología vegetal
- La epigenética es la epigenética.
- Biología molecular La biología molecular.
Sus antecedentes:
- La vernalización es un proceso crucial que permite a las plantas florecer en primavera después de la exposición al frío invernal.
- Esto implica el silenciamiento epigenético de los represores florales, en particular el LOCUS FLOWERING C (FLC) en Arabidopsis.
- El Complejo Represivo Polycomb 2 (PRC2) media este silenciamiento epigenético inducido por el frío.
Objetivo del estudio:
- Investigar el papel de los ARN no codificantes en el silenciamiento epigenético mediado por la vernalización del FLC.
- Identificar nuevos factores involucrados en la orientación de PRC2 al locus FLC durante la exposición al frío.
Principales métodos:
- Análisis de la expresión génica y las modificaciones de la cromatina en plantas Arabidopsis sometidas a tratamiento en frío.
- Los ensayos de inmunoprecipitación de ARN (RIP) y inmunoprecipitación de cromatina (ChIP) detectan las interacciones ARN-proteína y proteína-ADN.
- Análisis genético utilizando mutantes deficientes en ARN no codificantes específicos.
Principales resultados:
- Un ARN no codificante intrónico largo, llamado COLDAIR (ARN no codificante intrónico asistido por frío), se identifica como crítico para la vernalización.
- COLDAIR interactúa físicamente con una subunidad de PRC2.2.
- Se requiere COLDAIR para dirigir PRC2 al locus FLC, lo que lleva a una represión epigenética estable.
Conclusiones:
- COLDAIR es un componente clave en la vía de vernalización, actuando como una guía para PRC2 al gen FLC.
- Esta interacción establece una cromatina represiva estable en el FLC, asegurando el tiempo de floración adecuado en respuesta al frío invernal.
- COLDAIR representa un nuevo mecanismo para la regulación epigenética inducida por el medio ambiente en las plantas.
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