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Factor similar a la activina de una línea celular de Xenopus laevis responsable de la inducción del mesodermo
A J van den Eijnden-Van Raaij1, E J van Zoelent, K van Nimmen
1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Nature
|June 21, 1990
Resumen
Xenopus laevis ectoderm explants se puede inducir a formar mesodermo utilizando factores de crecimiento. Los investigadores identificaron que el factor inductor del mesodermo de Xenopus laevis (MIF) de las células XTC es el homólogo de la activina de mamíferos.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La inducción del mesodermo en la embriogénesis de anfibios es crucial para el desarrollo.
- Los factores de crecimiento como el factor de crecimiento transformador-beta (TGF-beta) pueden imitar este proceso in vitro.
- El factor específico de inducción del mesodermo (MIF) de las células XTC de Xenopus laevis sigue sin identificarse, a pesar de mostrar propiedades similares al TGF-beta.
Objetivo del estudio:
- Para investigar la naturaleza del factor inductor del mesodermo (MIF) producido por las células XTC de Xenopus laevis.
- Para analizar la relación entre la actividad MIF y la actividad de la activina en clones de células XTC.
- Para determinar si XTC-MIF es el homólogo de Xenopus de la activina de mamíferos.
Principales métodos:
- Aislamiento de líneas celulares homogéneas de poblaciones celulares XTC heterogéneas.
- Análisis de la producción del factor inductor del mesodermo (MIF) en diferentes clones de XTC.
- Medición de la actividad del factor de crecimiento transformador-beta (TGF-beta) y de la actividad similar a la activina en extractos celulares.
- Comparando la actividad de MIF con la actividad de activina a través de líneas celulares XTC.
Principales resultados:
- Solo un clon de XTC, XTC-GTX-11, produjo una actividad significativa de MIF.
- Este clon mostró una producción similar de factores de crecimiento conocidos, incluida la actividad de TGF-beta, en comparación con otros clones.
- Se observó una correlación clara y consistente entre la presencia de actividad MIF y la actividad de la activina en las células XTC y sus clones.
- La Activina A fue identificada como un potente factor inductor del mesodermo.
Conclusiones:
- El factor inductor del mesodermo (MIF) de las células XTC de Xenopus laevis está fuertemente asociado con la actividad de la activina.
- XTC-MIF se identifica como el homólogo de Xenopus de la activina de los mamíferos.
- Este hallazgo aclara la base molecular de la inducción del mesodermo en la embriogénesis de Xenopus laevis.
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