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La actividad inhibidora de la diferenciación se produce en formas asociadas a la matriz y difusibles que se generan
P D Rathjen1, S Toth, A Willis
1CRC Growth Factors Group, University of Oxford, England.
Cell
|September 21, 1990
Resumen
La diferenciación de las células madre embrionarias está regulada por la actividad inhibidora de la diferenciación / factor inhibidor de la leucemia (DIA / LIF). Este estudio revela que DIA/LIF existe tanto en formas difusibles como en formas ligadas a la matriz extracelular, lo que afecta el control del desarrollo.
Área de la Ciencia:
- Biología de las células madre Biología de las células madre
- Biología del desarrollo Biología del desarrollo.
- Biología molecular La biología molecular.
Sus antecedentes:
- La diferenciación de células madre embrionarias (CE) es un proceso crítico en el desarrollo.
- La actividad inhibidora de la diferenciación / factor inhibidor de la leucemia (DIA / LIF) es un regulador clave de la pluripotencia de las células ES.
- La supresión mediada por células alimentadoras de la diferenciación de células ES implica complejos mecanismos reguladores.
Objetivo del estudio:
- Para investigar los mecanismos reguladores de la diferenciación de las células ES.
- Comprender el papel de la localización DIA/LIF en el destino de las células ES.
- Para aclarar cómo las transcripciones alternas de DIA / LIF influyen en su función.
Principales métodos:
- Análisis de la diferenciación de las células ES en presencia de células alimentadoras.
- Investigación de la producción y localización de DIA/LIF.
- Examen de la expresión de transcripción alterna y su impacto en la estructura de las proteínas.
Principales resultados:
- DIA/LIF se produce como una proteína difusible y una forma inmovilizada asociada con la matriz extracelular.
- Las transcripciones alternativas que divergen en el exón 1 conducen a diferentes terminales amino.
- Estas transcripciones alternativas dirigen la incorporación de DIA/LIF biológicamente activo en la matriz extracelular.
Conclusiones:
- La doble localización de DIA/LIF (difusible e inmovilizada) es un hallazgo significativo.
- El uso alternativo del exón 1 es crucial para generar formas distintas de DIA/LIF.
- Esta producción de doble forma probablemente juega un papel vital en los mecanismos de control del desarrollo.
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