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Un intrón del grupo I de autoemplijamiento en el gen de la ADN polimerasa del bacteriófago SPO1 de Bacillus subtilis
H Goodrich-Blair1, V Scarlato, J M Gott
1Department of Biological Sciences, State University of New York, Albany 12222.
Cell
|October 19, 1990
Resumen
Los investigadores descubrieron un intrón de auto-espleaje en el bacteriófago SPO1 que infecta el Bacillus subtilis. Este hallazgo proporciona evidencia para el origen evolutivo temprano de intrones de auto-espleaje en bacterias.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
- Virología Virología.
Sus antecedentes:
- Los intrones autodesconectados son elementos genéticos capaces de separarse de las transcripciones de ARN.
- Los intrones del grupo IA son una clase bien caracterizada que se encuentra en los orgánulos eucariotas y en el bacteriófago T4.
- El bacteriófago SPO1 infecta a la bacteria gram-positiva Bacillus subtilis. también conocido como Bacillus subtilis.
Objetivo del estudio:
- Investigar la presencia y las características de los intrones en el bacteriófago SPO1.1.
- Para comparar el intrón SPO1 con intrones conocidos del grupo IA.
- Para explorar las implicaciones evolutivas de intrones de auto-espleaje en virus bacterianos.
Principales métodos:
- Análisis de la secuencia del genoma del bacteriófago SPO1.
- Predicción de la estructura secundaria del intrón.
- Identificación de la secuencia conservada y los motivos estructurales.
Principales resultados:
- Se identificó un intrón del grupo IA de auto-espleaje en el bacteriófago SPO1.1.
- El intrón SPO1 comparte características conservadas con los intrones IA del grupo T4 de eucariotas y bacteriófagos.
- El intrón contiene un marco de lectura abierto que codifica la ADN polimerasa similar a la ADN polimerasa I de E. coli.
Conclusiones:
- Los intrones autodireccionados están presentes en los virus que infectan tanto a las bacterias gram-positivas como a las gram-negativas.
- Este hallazgo apoya un origen temprano de los intrones en la evolución de la vida.
- El intrón SPO1 proporciona un nuevo modelo para estudiar la evolución y la función de los intrones.
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