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In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples
Published on: August 31, 2018
Ciclos complejos de diel de la expresión génica en la simbiosis coral-alga
1Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.
Science (New York, N.Y.)
|January 15, 2011
Resumen
Los relojes circadianos de los corales los preparan para el estrés oxidativo de la fotosíntesis del simbionte mediante la regulación de los genes del estrés. Sin embargo, el metabolismo central está controlado por factores inducibles a la hipoxia, lo que revela una compleja regulación de la endosimbiosis.
Área de la Ciencia:
- Biología marina Biología marina.
- Cronobiología cronobiología.
- La investigación de la simbiosis es la investigación de la simbiosis.
Sus antecedentes:
- Las endosimbiosis planta-animal, como las simbiosis coral-alga, están influenciadas por diversas señales ambientales e internas.
- El reloj circadiano juega un papel en la coordinación de los procesos biológicos dentro de los organismos.
Objetivo del estudio:
- Para investigar el acoplamiento del reloj circadiano del coral a los genes relacionados con el estrés.
- Para entender cómo la regulación circadiana de los corales se prepara para el estrés oxidativo de los simbiontes de algas.
Principales métodos:
- Análisis de los patrones de expresión génica en los corales.
- Investigando la relación entre los ritmos circadianos y los genes de respuesta al estrés.
- Examinando el papel del sistema de factores inducibles por hipoxia en el metabolismo de los corales.
Principales resultados:
- Los genes relacionados con el estrés de los corales, incluidas las chaperonas y los genes antioxidantes, están sincronizados con el reloj circadiano.
- La expresión génica controlada por el reloj anticipa el estrés oxidativo causado por los altos niveles de oxígeno durante la fotosíntesis del simbionte.
- El metabolismo central en los corales parece estar regulado por el sistema de factores inducibles por hipoxia, independiente del reloj circadiano.
Conclusiones:
- La regulación del reloj circadiano de los corales es compleja, con vías distintas para la respuesta al estrés y el metabolismo central.
- El reloj circadiano prepara a los corales para el estrés oxidativo inducido por el simbionte, destacando la plasticidad adaptativa.
- La regulación de la endosimbiosis implica interacciones intrincadas entre el reloj del huésped y las señales ambientales.
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