Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

Circadian Rhythms and Gene Regulation

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

Biological Clocks and Seasonal Responses

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Chronopharmacokinetics: Circadian Rhythms and Influence on Drug Response

Circadian rhythms are cyclic changes that are crucial in plasma drug concentrations. Various standard circadian parameters, including core body temperature, heart rate, and other cardiovascular factors, directly impact disease states and the therapeutic response to drug therapy.
The time of drug administration is an important factor to consider, as it can influence the toxic dose of a drug. For example, a study conducted by Prins et al. in 1997 examined the effects of the timing of...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Divergent signaling profiles in mTOR gain-of-function Smith-Kingsmore syndrome (SKS) and TSC2 deficiency.

bioRxiv : the preprint server for biology·2026

Same author

Circadian rhythm defects in Prader-Willi syndrome neurons.

HGG advances·2025

Same author

AAV8-P301L tau expression confers age-related disruptions in sleep quantity and timing.

Npj biological timing and sleep·2024

Same author

Clinical and functional studies of MTOR variants in Smith-Kingsmore syndrome reveal deficits of circadian rhythm and sleep-wake behavior.

HGG advances·2024

Same author

Lifelong Chronic Sleep Disruption in a Mouse Model of Traumatic Brain Injury.

Neurotrauma reports·2024

Same author

Sleep Disruption in a Mouse Model of Chronic Traumatic Brain Injury.

bioRxiv : the preprint server for biology·2023

Search research articles

Video Experimental Relacionado

Updated: Jun 5, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

Se requiere un retraso en la represión de retroalimentación por el criptocromo 1 para la función del reloj

Maki Ukai-Tadenuma¹, Rikuhiro G Yamada, Haiyan Xu

¹Laboratory for Systems Biology, RIKEN Center for Developmental Biology, Chuo-ku, Kobe, Hyogo, Japan.

|January 18, 2011

Resumen

Retrasar la expresión del criptocromo 1 (Cry1) es crucial para que el reloj circadiano de los mamíferos funcione correctamente. Esta investigación demuestra cómo elementos específicos del ADN controlan el Cry1.

Más Videos Relacionados

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

Videos de Experimentos Relacionados

Last Updated: Jun 5, 2026

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

Published on: September 17, 2016

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Published on: July 4, 2018

Área de la Ciencia:

Cronobiología cronobiología.
Biología Molecular Biología Molecular
Genética La genética.

Sus antecedentes:

El reloj circadiano de los mamíferos regula los ritmos biológicos diarios.
La represión de la retroalimentación es esencial para la función del reloj, pero ha faltado evidencia directa.
El criptocromo 1 (Cry1) es una proteína clave involucrada en la regulación del ritmo circadiano.

Objetivo del estudio:

Para investigar el requisito de la represión de retroalimentación retardada en el reloj circadiano de los mamíferos.
Aclarar los mecanismos reguladores que subyacen a la expresión nocturna del criptocromo 1 (Cry1).
Determinar el papel del tiempo de expresión de Cry1 en el mantenimiento de la ritmicidad circadiana.

Principales métodos:

Análisis de los elementos reguladores del gen Cry1, incluidas las cajas D y las RRE.
Construcción y prueba de un promotor de compuesto sintético para Cry1.
Desarrollo y aplicación de un modelo de vector de fase.
Ensayos de complementación genética en las células Cry1(-/-):Cry2(-/-.

Principales resultados:

Una combinación de elementos diurnos (caja D) y nocturnos (RREs) impulsa la expresión Cry1 nocturna.
Un promotor sintético recapituló este patrón de expresión.
Se observó la modulación del retraso de fase mediante la coordinación de estos elementos.
El retraso sustancial en la expresión de Cry1 fue necesario para restaurar la ritmicidad circadiana en las células knockout.
El retraso prolongado de Cry1 condujo a una desaceleración de las oscilaciones circadianas.

Conclusiones:

El retraso de fase en la transcripción del criptocromo 1 (Cry1) es un requisito crítico para la función del reloj de los mamíferos.
La interacción entre los elementos promotores y potenciadores dicta el tiempo de expresión de Cry1.
El tiempo preciso de la expresión de Cry1 influye en la velocidad de las oscilaciones circadianas.