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Los relojes circadianos en los glóbulos rojos humanos
John S O'Neill1, Akhilesh B Reddy
1Department of Clinical Neurosciences, University of Cambridge Metabolic Research Laboratories, Institute of Metabolic Science, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK.
Nature
|January 29, 2011
Resumen
Los glóbulos rojos humanos revelan que la transcripción no es necesaria para los ritmos circadianos. Los mecanismos no transcripcionales, como los ciclos redox de peroxiredoxina, sostienen los relojes circadianos celulares, desafiando los modelos existentes.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Cronobiología cronobiología.
- Biología celular Biología celular.
Sus antecedentes:
- Los relojes circadianos regulan los procesos fisiológicos en diversos organismos.
- Los modelos actuales de mamíferos se basan en circuitos de retroalimentación de transcripción-traducción.
- El estudio de los mecanismos de reloj no transcripcionales en los mamíferos es un desafío.
Objetivo del estudio:
- Investigar los mecanismos no transcripcionales de los ritmos circadianos en las células humanas.
- Para determinar si la transcripción es esencial para las oscilaciones circadianas celulares.
- Para explorar el papel de las peroxiredoxinas en la regulación circadiana.
Principales métodos:
- Desarrolló nuevos ensayos utilizando glóbulos rojos humanos (que carecen de núcleo).
- Ciclos redox de peroxiredoxina monitoreados a lo largo del tiempo en condiciones constantes.
- Evaluó la entrainabilidad y la compensación de temperatura de los ritmos observados.
Principales resultados:
- Se observaron oscilaciones circadianas en los ciclos redox de peroxiredoxina en los glóbulos rojos humanos.
- Estos ritmos persistieron durante días sin señales externas y fueron compensados por la temperatura.
- Se descubrió que la transcripción no es necesaria para mantener estos ritmos circadianos celulares.
Conclusiones:
- Los eventos no transcripcionales son suficientes para mantener los ritmos circadianos celulares en los seres humanos.
- Los ciclos redox de peroxiredoxina representan un mecanismo circadiano no transcripcional clave.
- Los hallazgos sugieren un papel más amplio para las oscilaciones no transcripcionales en los relojes circadianos eucariotas.
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