Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Assessing the contribution of rare DNA states to cancer mutational signatures using sequence-specific conformational fingerprinting.

Nature communications·2026
Same author

Thermodynamic prediction of RNA cellular activity from sequence via conformational ensembles.

Cell·2026
Same author

Revealing hidden protonated conformational states in RNA dynamic ensembles.

Nucleic acids research·2025
Same author

Recurrent Neural Networks Predict Future Peptide Aggregation for Drug Development.

Molecular pharmaceutics·2025
Same author

Enabling Liquid Scanning Electron Microscopy for Therapeutic Suspensions Using Vacuum-Compatible Liquid Capsules.

Analytical chemistry·2025
Same author

Probing rare and short-lived conformational states in nucleic acids using off-resonance carbonyl and guanidino carbon R<sub>1ρ</sub> relaxation dispersion.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2025

Video Experimental Relacionado

Updated: Jun 4, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

La flexibilidad de la secuencia específica del B-ADN modula la formación del Z-ADN.

Jameson R Bothe1, Ky Lowenhaupt, Hashim M Al-Hashimi

  • 1Department of Chemistry and Biophysics, University of Michigan, Ann Arbor, Michigan 48109, United States.

Journal of the American Chemical Society
|February 1, 2011
PubMed
Resumen
Este resumen es generado por máquina.

Las transiciones estructurales del ADN, como el ADN-B al ADN-Z, son cruciales para la regulación de los genes. Este estudio revela cómo la flexibilidad del ADN influye en la formación de Z-ADN y la ubicación de la unión B / Z, con CG-repeticiones que juegan un papel clave.

Más Videos Relacionados

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Videos de Experimentos Relacionados

Last Updated: Jun 4, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • La biofísica es la biofísica.
  • Genética La genética.

Sus antecedentes:

  • La transición entre el B-ADN diestro y el Z-ADN zurdo es un cambio estructural significativo en la biología.
  • La formación de ADN-Z es esencial para la expresión y regulación génica, pero requiere de costosas uniones B/Z energéticas.

Objetivo del estudio:

  • Investigar cómo la flexibilidad de la secuencia específica de B-ADN afecta la propensión termodinámica para la formación de Z-ADN.
  • Determinar el papel de la flexibilidad del ADN-B en la localización de las uniones B/Z.

Principales métodos:

  • Abundancia natural RMN R(1ρ) mediciones de la relajación del carbono.
  • Espectroscopía de dicroísmo circular (CD). espectroscopía de dicroísmo circular (CD). espectroscopía de dicroísmo circular (CD). espectroscopía de dicroísmo circular (CD). espectroscopía de dicroísmo circular (CD). espectroscopía de dicroísmo circular (CD).

Principales resultados:

  • La flexibilidad específica de la secuencia B-ADN, observada en escalas de tiempo rápidas (ps-ns) y lentas (micros-ms), modula la formación de Z-ADN.
  • Esta flexibilidad se localiza en los puntos de unión B/Z.
  • Las repeticiones de CG ajustan activamente la flexibilidad intrínseca del ADN-B.

Conclusiones:

  • La flexibilidad de la secuencia específica del B-ADN es un factor clave que influye en la formación del Z-ADN y el posicionamiento de las uniones dentro de los genomas.
  • Esta flexibilidad puede servir como un mecanismo de regulación para controlar la longitud y la ubicación del ADN-Z.