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Las divisiones celulares asimétricas promueven la diferenciación epidérmica dependiente de Notch
Scott E Williams1, Slobodan Beronja, H Amalia Pasolli
1Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, New York 10065, USA.
Nature
|February 19, 2011
Resumen
Las divisiones de las células madre controlan el desarrollo del tejido. Este estudio revela cómo la regulación de la orientación del husillo en las células progenitoras de la piel del ratón es crucial para la correcta estratificación de la piel y la formación de barreras.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- La ingeniería de tejidos.
Sus antecedentes:
- Las células madre y progenitoras se dividen asimétricamente para equilibrar la proliferación y la diferenciación.
- Los estudios en invertebrados identificaron complejos de proteínas que regulan la polaridad celular y el posicionamiento del huso durante la mitosis.
Objetivo del estudio:
- Investigar el papel de la orientación del husillo en la regulación de las divisiones celulares asimétricas durante el desarrollo de la piel del ratón.
- Determinar la participación de LGN, NuMA y dynactina en este proceso.
- Comprender los efectos posteriores de las divisiones asimétricas comprometidas en la arquitectura de la piel.
Principales métodos:
- Utilizado in vivo la interferencia del ARN lentiviral específico de la piel en modelos de ratón.
- Defectos evaluados en la estratificación, diferenciación y formación de barreras.
- Investigó el papel de la señalización Notch como un efector aguas abajo.
Principales resultados:
- Proporcionó evidencia directa de la participación de LGN (Gpsm2), NuMA y dynactina (Dctn1) en la regulación de la orientación del husillo en los progenitores de la piel del ratón.
- Se demostró que comprometer las divisiones celulares asimétricas conduce a graves defectos en la estratificación, diferenciación y función de barrera de la piel.
- La señalización de Notch implícita como una vía clave afectada por estos defectos.
Conclusiones:
- La orientación del husillo es fundamental para establecer una arquitectura de tejido adecuada durante el desarrollo de la piel de los mamíferos.
- LGN, NuMA y dynactina son reguladores clave de la división celular asimétrica en los progenitores de la piel.
- La interferencia de ARN in vivo es una herramienta eficaz para estudiar las complejas interacciones genéticas en el desarrollo de los mamíferos.
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