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Updated: Jun 3, 2026

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

Sobre la estereoquímica del 2-hidroxietilfosfonato dioxigenasa

John T Whitteck1, Petra Malova, Spencer C Peck

  • 1Howard Hughes Medical Institute and Roger Adams Laboratory, Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|March 9, 2011
PubMed
Resumen

La enzima HEPD convierte el 2-hidroxietilfosfonato en hidroximetilfosfonato mediante la eliminación de un hidrógeno específico. Este proceso pierde inesperadamente la información estereoquímica en C1, lo que requiere una reevaluación de los mecanismos enzimáticos conocidos.

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