Video Experimental Relacionado
Updated: Jun 3, 2026
10:01
Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
La conmutación eléctrica entre las vesículas y las micelas a través del autoensamblaje sensible al redox de las
Hoon Kim1, Sang-Mi Jeong, Ji-Woong Park
1Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Korea.
Journal of the American Chemical Society
|March 23, 2011
Resumen
Los campos eléctricos controlan las vesículas de autoensamblaje. La tensión oxidante divide las vesículas en micelas, mientras que la reducción de la tensión las reforma, lo que demuestra un nuevo sistema de respuesta redox sin aditivos químicos.
Área de la Ciencia:
- Química supramolecular de las moléculas.
- Ciencia de los materiales Ciencia de los materiales.
- La electroquímica es electroquímica.
Sus antecedentes:
- Los sistemas vesiculares son cruciales en la administración de medicamentos y en la nanotecnología.
- El control del autoensamblaje con estímulos externos es un desafío clave.
- Los métodos existentes a menudo requieren agentes redox químicos.
Objetivo del estudio:
- Para demostrar un sistema vesicular acuoso eléctricamente conmutable.
- Para utilizar el autoensamblaje de respuesta redox para la manipulación de las vesículas.
- Para evitar los agentes redox químicos en el proceso de conmutación.
Principales métodos:
- Sintetizó una molécula de rod-coil anfifílico con bloques de tetraanilina y poli (etilenglicol).
- Se ha investigado el autoensamblaje redox-responsivo en solución acuosa.
- Aplica voltajes oxidantes y reductores para inducir cambios estructurales.
Principales resultados:
- Desarrolló un sistema vesicular conmutable controlado por el potencial eléctrico.
- La tensión oxidante indujo el desmontaje de las vesículas en micelas parecidas a un disco.
- La reducción de la tensión desencadenó el reensamblaje de micelas de nuevo en vesículas.
Conclusiones:
- El estado de oxidación del bloque de tetraanilina rige el comportamiento de embalaje y la estabilidad de la membrana.
- Este sistema ofrece un método sin químicos para el control dinámico de estructuras autoensambladas.
- Aplicaciones potenciales en materiales sensibles y sistemas de liberación controlada.
Videos de Conceptos Relacionados
01:32Pinching-off of Coated Vesicles
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
01:12Clathrin Coated Vesicles
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
01:26Fusion of Secretory Vesicles with the Plasma Membrane
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
01:45Vesicular Tubular Clusters
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
01:38Intralumenal Vesicles and Multivesicular Bodies
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
01:43SNAREs and Membrane Fusion
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...