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Evidencia de que el gen transformador del virus del sarcoma aviar codifica una proteína quinasa asociada con una

A D Levinson, H Oppermann, L Levintow

|October 1, 1978

Resumen

El oncogén src del virus del sarcoma aviar (VSA) codifica una fosfoproteína de 60.000 daltones, pp60. Esta proteína exhibe actividad quinasa, lo que sugiere que su fosforilación es crucial para la transformación celular inducida por ASV.

Área de la Ciencia:

Biología Molecular Biología Molecular
Virología Virología.
Oncología Oncología.

Sus antecedentes:

El virus del sarcoma aviar (VSA) causa tumores y transforma las células a través de su gen src.
Una proteína de 60.000 daltones, potencialmente codificada por src, está implicada en la transformación neoplásica.

Objetivo del estudio:

Para aislar y caracterizar el producto putativo del gen src.
Para investigar la función y el origen genético de la proteína de 60.000 daltones.

Principales métodos:

Inmunoprecipitación con el uso de antisera de conejos portadores de tumores ASV.
Análisis bioquímico y genético de la proteína aislada.
Ensayos de fosforilación in vitro y análisis de mutantes sensibles a la temperatura.

Principales resultados:

Se identificó y confirmó que una fosfoproteína de 60,000 daltones, pp60, estaba codificada por src.
pp60 posee actividad intrínseca de proteína quinasa, fosforilación de las cadenas pesadas de inmunoglobulina.
La fosforilación de pp60 fue sensible a la temperatura, correlacionándose con la función src.

Conclusiones:

pp60 y su actividad asociada de proteína quinasa están codificadas por el gen ASV src.
La fosforilación de pp60 es probablemente esencial para su papel en la transformación neoplásica.
La quinasa responsable de la fosforilación de pp60 puede diferir de la quinasa codificada por src.

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