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Updated: Jun 3, 2026

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Published on: July 3, 2016

Enfoque sintético para detener la mutagenesis por escaneo de codón de mutagenesis.

Lihua Nie1, Jason J Lavinder, Mohosin Sarkar

  • 1Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.

Journal of the American Chemical Society
|April 2, 2011
PubMed
Resumen

Un nuevo método permite el escaneo genético controlado utilizando codones de parada ámbar para insertar varios aminoácidos. Esta técnica reveló interacciones proteicas inesperadas y la formación potencial de tetrámeros en la proteína Rop.

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