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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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Argonaute10 como un almacenador de miRNA.

Pablo A Manavella1, Detlef Weigel, Liang Wu

  • 1Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tübingen, Germany.

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|April 19, 2011
PubMed
Resumen

Las plantas usan proteínas argonautas para secuestrar microARN (miRNA), impidiéndoles regular la estabilidad y la traducción del ARN mensajero. Este estudio revela un nuevo mecanismo regulador en las plantas que involucra la unión miRNA-Argonaute.

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Ciencias de las plantas Ciencias de las plantas.

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  • Regulación genética Reglamento genético.

    • Sus antecedentes:

    • Los microARN (miRNA) son reguladores clave de la expresión génica, que influyen en la estabilidad del ARN mensajero (ARNm) y la eficiencia de la traducción.
    • Las proteínas argonautas (AGO) son componentes esenciales del complejo de silenciamiento inducido por ARN (RISC), que media la función del miARN.
    • Comprender la regulación génica mediada por miRNA es crucial para el desarrollo de las plantas y las respuestas al estrés.