Los factores de crecimiento inducen la fosforilación del antiportador Na+/H+, una glicoproteína de 110 kD
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A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
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C Sardet1, L Counillon, A Franchi
1Centre de Biochimie-CNRS, Nice, France.
Science (New York, N.Y.)
|February 9, 1990
Resumen
El estudio identificó el antiportador Na+/H+ como una glicoproteína fosforilada. Su fosforilación aumenta con la estimulación mitogénica, lo que sugiere un papel en la regulación del pH intracelular.
Área de la Ciencia:
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El antiportador Na+/H+ regula el pH intracelular en todas las células.
- Su actividad se altera rápidamente por la activación mitogénica y oncogénica, pero el mecanismo es desconocido.
Objetivo del estudio:
- Para investigar el mecanismo molecular de la activación del antiportador Na+/H+.
Principales métodos:
- Expresó un fragmento de cDNA antiportador Na+/H+ en E. coli como una proteína de fusión.
- Desarrolló un anticuerpo específico contra la proteína de fusión.
- Utilizó el anticuerpo para detectar y caracterizar el antiportador en células de mamíferos.
Principales resultados:
- Se identificó el antiportador Na+/H+ como una glicoproteína de 110 kilodaltones.
- Se demostró que el antiportador se fosforila en las células en crecimiento.
- Se demostró que la activación mitogénica estimula la fosforilación del antiportador, en correlación con el aumento del pH intracelular.
Conclusiones:
- El antiportador Na+/H+ es una glicoproteína cuya fosforilación está modulada por señales mitogénicas.
- Esta fosforilación probablemente juega un papel en los rápidos cambios en la actividad antiportadora observada tras la estimulación.
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