Video Experimental Relacionado
Updated: May 13, 2026
10:15
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA
Published on: July 6, 2012
La ARN polimerasa II en pausa como punto de control del desarrollo
1Division of Genetics, Genomics, and Development, Department of Molecular and Cell Biology, Center for Integrative Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|May 14, 2011
Resumen
La activación génica a menudo se basa en la liberación de la ARN polimerasa II (Pol II) del promotor, no solo en la unión inicial. Esto desafía la visión de los libros de texto e impacta la comprensión de las redes de genes del desarrollo.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- El modelo tradicional postula la interacción del promotor de la ARN polimerasa II (Pol II) como el paso que limita la velocidad en la activación del gen.
- La evidencia emergente sugiere que los mecanismos reguladores alternativos son críticos para el control de la expresión génica.
Objetivo del estudio:
- Revisar el mecanismo de activación génica centrándose en el alargamiento de la transcripción.
- Discutir las implicaciones de la liberación de Pol II del promotor proximal en la regulación de la red génica durante el desarrollo.
Principales métodos:
- Revisión de la literatura de estudios sobre la activación génica y la regulación de la transcripción.
- Análisis de datos de células madre embrionarias humanas y embriones de Drosophila.
- Síntesis de los hallazgos relacionados con la liberación de Pol II y la dinámica de la red génica.
Principales resultados:
- La evidencia desafía la visión del libro de texto de que la activación génica está limitada únicamente a la interacción del promotor.
- La regulación de la elongación de la transcripción, específicamente la liberación de Pol II, es cada vez más reconocida como crucial para la expresión génica diferencial.
- Este mecanismo juega un papel en las complejas redes de genes que controlan el desarrollo de los animales.
Conclusiones:
- La liberación de Pol II del promotor proximal es un paso regulador clave en la activación génica.
- Comprender este mecanismo es vital para descifrar el control genético del desarrollo animal.
- Esta perspectiva revisada tiene un impacto en el estudio de las redes genéticas y los procesos de desarrollo.
Más Videos Relacionados
10:59Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
10:49A Murine Cell Line Based Model of Chronic CDK9 Inhibition to Study Widespread Non-Genetic Transcriptional Elongation Defects (TEdeff) in Cancers
Published on: September 26, 2019
Videos de Conceptos Relacionados
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
00:58Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
00:58Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...