Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Test of CP Symmetry in the Neutral Decays of Λ via J/ψ→ΛΛ[over ¯].

Physical review letters·2026
Same author

[Progress in basic research on hydrogel-based drug delivery systems for the treatment of peri-implantitis].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

Precise Measurement of the Chromoelectric Dipole Moment of the Charm Quark.

Physical review letters·2026
Same author

First Measurement of the D_{s}^{+}→K^{0}μ^{+}ν_{μ} Decay.

Physical review letters·2026
Same author

Observation of the Electromagnetic Radiative Decays of the Λ(1520) and Λ(1690) to γΣ^{0}.

Physical review letters·2026
Same author

Observation of a Threshold Enhancement in the π^{+}π^{-} Spectrum in ψ(3686)→π^{+}π^{-}J/ψ Decays.

Physical review letters·2026

Video Experimental Relacionado

Updated: Jun 2, 2026

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

Mapeo de orientación tridimensional en el microscopio electrónico de transmisión.

H H Liu1, S Schmidt, H F Poulsen

  • 1Center for Fundamental Research: Metal Structures in Four Dimensions, Materials Research Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, DK-4000 Roskilde, Denmark.

Science (New York, N.Y.)
|May 14, 2011
PubMed
Resumen
Este resumen es generado por máquina.

Los investigadores desarrollaron una nueva técnica no destructiva para el mapeo 3D de orientación de grano en materiales nanocristalinos. Este método logra una resolución espacial de 1 nm utilizando un microscopio electrónico de transmisión, superando las técnicas anteriores.

Más Videos Relacionados

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples
09:17

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

Videos de Experimentos Relacionados

Last Updated: Jun 2, 2026

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography
08:15

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Published on: June 9, 2018

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples
09:17

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

Published on: August 6, 2025

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy
09:12

Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

Published on: June 9, 2022

Área de la Ciencia:

  • Ciencia de los materiales Ciencia de los materiales.
  • La cristalografía es una técnica de cristalografía.
  • El microscopio de la microscopía.

Sus antecedentes:

  • El desarrollo de técnicas no destructivas para el mapeo de orientación de grano en 3D es crucial para comprender los materiales cristalinos.
  • Los métodos existentes como la microscopía de difracción de rayos X 3D ofrecen resoluciones de hasta 200 nm.

Objetivo del estudio:

  • Introducir una nueva técnica no destructiva para el mapeo de orientación de grano 3D de alta resolución.
  • Para permitir el análisis microstructural detallado de materiales nanocristalinos monofásicos y multifásicos.

Principales métodos:

  • Utilizó microscopía electrónica de transmisión (TEM) para el mapeo de orientación en 3D.
  • Se logró una resolución espacial de 1 nm.
  • Valida la técnica a través de estudios experimentales en aluminio nanocristalino y simulaciones.

Principales resultados:

  • Se ha demostrado el mapeo 3D de orientación de grano con resolución espacial de 1 nm en materiales nanocristalinos.
  • Mapeó con éxito las orientaciones de los granos en muestras monofásicas y multifásicas.
  • Las simulaciones confirmaron los principios y la eficacia de la técnica desarrollada.

Conclusiones:

  • La nueva técnica basada en TEM avanza significativamente la capacidad para el análisis microstructural 3D a nanoescala.
  • Este método proporciona un detalle sin precedentes para el estudio de materiales nanocristalinos.
  • La técnica está validada y es aplicable a varios sistemas de materiales nanocristalinos.