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Iniciación del patrón proximal-distal en el miembro de los vertebrados por señales y crecimiento
Kimberly L Cooper1, Jimmy Kuang-Hsien Hu, Derk ten Berge
1Harvard Medical School, Department of Genetics, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
Science (New York, N.Y.)
|May 28, 2011
Resumen
El desarrollo temprano de las extremidades de los pollitos revela que el mesénquima puede formar todos los segmentos. El patrón proximal depende de las señales de los flancos, mientras que los segmentos medial y distal se desarrollan más allá de esta influencia.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Morfogénesis de extremidades Morfogénesis de extremidades
- Embriología de los vertebrados.
Sus antecedentes:
- El eje proximal-distal de las extremidades de los vertebrados es crucial para la formación de las extremidades.
- Los modelos existentes proponen diferentes mecanismos para el patrón de las extremidades.
- Comprender el desarrollo de segmentos de extremidades es clave para la biología del desarrollo.
Objetivo del estudio:
- Para diferenciar entre los modelos propuestos de patrón de eje proximal-distal de las extremidades.
- Investigar el papel del mesénquima de las extremidades tempranas en la generación de todos los segmentos de las extremidades.
- Para dilucidar los mecanismos de señalización subyacentes al desarrollo de segmentos de extremidades.
Principales métodos:
- Utilizó un sistema de cultivo combinado in vitro e in vivo.
- Analizó el desarrollo del mesénquima de las extremidades de pollo.
- Investigó la influencia de la señalización proximal y distal en el patrón de las extremidades.
Principales resultados:
- El mesénquima de las extremidades tempranas del pollo es inicialmente plástico, capaz de formar todos los segmentos.
- El patrón de los miembros proximales se mantiene mediante señales continuas derivadas de los flancos.
- El desarrollo del segmento de extremidad medial y distal se inicia en regiones que crecen más allá de la influencia de la señalización proximal.
Conclusiones:
- El estudio proporciona evidencia que apoya un modelo en el que el mesénquima de las extremidades es inicialmente equipotente.
- El patrón de los miembros implica una transición de la dependencia de la señal proximal a los programas de desarrollo intrínsecos.
- El sistema de cultivo desarrollado ofrece nuevas vías para estudiar los mecanismos de patronaje de las extremidades.
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