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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...

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Updated: Jul 20, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation
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Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Múltiples proteínas específicas del tipo celular regulan diferencialmente el reconocimiento de la secuencia objetivo

C K Glass1, O V Devary, M G Rosenfeld

  • 1Department of Medicine, University of California, San Diego School of Medicine, La Jolla 92093-0648.

Cell
|November 16, 1990
PubMed
Resumen

Múltiples proteínas nucleares, denominadas correguladores del receptor del ácido retinoico, modulan la actividad de transcripción génica del receptor del ácido alfa retinoico. Estos correguladores ajustan las funciones de los receptores de ácido retinoico durante el desarrollo de los vertebrados.

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Área de la Ciencia:

  • Biología del desarrollo Biología del desarrollo.
  • Endocrinología Molecular La Endocrinología Molecular
  • Regulación genética Reglamento genético.

Sus antecedentes:

  • Los receptores de ácido retinoico (RAR) son cruciales para el desarrollo de los vertebrados, controlando la transcripción génica de una manera específica para cada tipo de célula.
  • Comprender los mecanismos precisos por los cuales los RAR regulan la expresión génica es esencial para descifrar los procesos de desarrollo.

Objetivo del estudio:

  • Para investigar la existencia y la función de las proteínas nucleares que modulan la afinidad de unión del receptor de ácido alfa retinoico (RARα).
  • Aclarar el papel de estas nuevas proteínas en la dirección de la actividad transcripcional RAR durante el desarrollo.

Principales métodos:

  • Investigó la interacción entre RARα y las proteínas nucleares específicas del tipo de célula.
  • Se analizó el efecto de estas proteínas en la unión de RARα a los elementos de respuesta.
  • Caracterizó la interfaz involucrada en la interacción entre RARα y las proteínas correguladoras.

Principales resultados:

  • La evidencia apoya la existencia de múltiples proteínas nucleares específicas del tipo de célula que actúan como correguladores de RAR.
  • Estos correguladores mejoran la afinidad de unión de RARα a varios elementos de respuesta.
  • La interacción del corregulador ocurre en una interfaz de dimerización que se superpone al dominio de unión de ligando RARα.

Conclusiones:

  • Las proteínas correguladoras específicas del tipo de célula modulan diferencialmente la actividad transcripcional RARα.
  • Las proteínas correguladoras pueden restringir o dirigir los efectos RAR en los patrones de expresión génica durante el desarrollo.
  • Esta interacción proporciona un mecanismo para ajustar la señalización del ácido retinoico en el desarrollo.