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La quiralidad en la forma de las células planas contribuye a la morfogénesis epitelial asimétrica izquierda-derecha
Kiichiro Taniguchi1, Reo Maeda, Tadashi Ando
1Department of Biological Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Science (New York, N.Y.)
|July 19, 2011
Resumen
Los órganos de los animales muestran asimetría izquierda-derecha. En Drosophila, la quiralidad de la forma de las células planas (PCC) impulsa la rotación del intestino posterior, revelando la quiralidad celular intrínseca como un mecanismo clave en el desarrollo de tejidos asimétricos.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- La biofísica es la biofísica.
Sus antecedentes:
- Los organismos exhiben asimetría izquierda-derecha (LR) en varios órganos.
- Comprender los mecanismos de la morfogénesis asimétrica de LR es crucial para la biología del desarrollo.
- El tubo epitelial del intestino posterior de Drosophila proporciona un sistema modelo para estudiar la morfogénesis asimétrica de LR.
Objetivo del estudio:
- Para investigar el papel de la quiralidad de la forma de las células planas (PCC) en la rotación direccional LR de la Drosophila hindgut.
- Aclarar los mecanismos moleculares subyacentes a la PCC y su contribución a la morfogénesis tisular.
Principales métodos:
- Observación de la rotación direccional de LR en el tubo epitelial del intestino posterior de Drosophila.
- Análisis de la distribución de Drosophila E-cadherin (DE-Cad) y su asimetría LR.
- Investigando la función de la miosina ID en la regulación de la polaridad de LR.
- Simulación in silico para evaluar la suficiencia del PCC para inducir la rotación del tejido.
Principales resultados:
- Las células epiteliales del intestino trasero exhiben formas asimétricas (quirales) LR, denominadas quiralidad de la forma de las células planas (PCC).
- La distribución de Drosophila E-cadherin (DE-Cad) muestra una asimetría LR, lo que contribuye a la PCC.
- El ID de miosina influye en la polaridad de LR tanto en la distribución PCC como en la DE-Cad, correlacionándose con la dirección de rotación.
- Los modelos in silico confirman la suficiencia del PCC para impulsar la rotación direccional de los tejidos.
Conclusiones:
- La quiralidad celular intrínseca, específicamente PCC, es un mecanismo fundamental que impulsa la morfogénesis de tejido asimétrico LR.
- La asimetría DE-Cad y la actividad de la miosina ID son reguladores clave de la PCC y la rotación del intestino trasero.
- Este estudio proporciona información sobre la base celular de la asimetría izquierda-derecha en los órganos en desarrollo.
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