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Las nectinas establecen un patrón celular en forma de tablero de ajedrez en el epitelio auditivo
Hideru Togashi1, Kanoko Kominami, Masazumi Waseda
1Division of Molecular and Cellular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan.
Science (New York, N.Y.)
|July 30, 2011
Resumen
Las moléculas de adhesión celular de la nectina-1 y la nectina-3 son cruciales para la disposición precisa del tablero de ajedrez de las células ciliadas sensoriales y las células de apoyo en la cóclea. Su interacción asegura el espaciamiento celular adecuado y evita conexiones anormales.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- La neurociencia auditiva es una neurociencia auditiva.
Sus antecedentes:
- El epitelio auditivo de la cóclea exhibe una precisa disposición en forma de tablero de ajedrez de las células ciliadas sensoriales y las células de soporte.
- Los mecanismos moleculares que gobiernan este patrón celular específico siguen siendo en gran medida desconocidos.
Objetivo del estudio:
- Investigar el papel de las moléculas de adhesión similares a las inmunoglobulinas en el establecimiento del patrón del tablero de ajedrez de los epitelios auditivos.
- Para identificar las moléculas específicas que median la adhesión heterotípica entre las células ciliadas y las células de apoyo.
Principales métodos:
- Utilizó modelos de ratón para estudiar la expresión y función de las moléculas de adhesión de la nectina.
- Investigó los efectos de la eliminación genética de la nectina-1 y la nectina-3 en el arreglo celular.
- Realizó experimentos de co-cultivo con células que expresan nectina-1 o nectina-3.
Principales resultados:
- Las células ciliadas y las células de apoyo en la cóclea expresan nectina-1 y nectina-3, respectivamente.
- La deleción genética de la nectina-1 o la nectina-3 interrumpió el patrón del tablero de ajedrez, lo que condujo a anexos anormales de células ciliadas.
- Las células co-cultivadas que expresan nectina-1 o nectina-3 formaron un patrón de mosaico, lo que indica su papel en el reconocimiento y espaciado celular.
Conclusiones:
- La nectina-1 y la nectina-3 median la adhesión heterotípica entre las células ciliadas y las células de soporte.
- Estas moléculas de nectina son esenciales para formar el patrón característico en forma de tablero de ajedrez en el epitelio auditivo.
- Los hallazgos aclaran un mecanismo clave en el desarrollo de las estructuras sensoriales en el oído interno.
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