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Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...

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Video Experimental Relacionado

Updated: May 29, 2026

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

Las chaperoninas facilitan el tráfico KNOTTED1 de célula a célula y la función de las células madre.

Xianfeng Morgan Xu1, Jing Wang, Zhenyu Xuan

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Science (New York, N.Y.)
|August 27, 2011
PubMed
Resumen

Los complejos de chaperonina son vitales para el mantenimiento de las células madre de las plantas al facilitar el tráfico de célula a célula de los factores de transcripción KNOTTED1 (KN1) homeobox (KNOX) a través de plasmodesmata.

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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
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In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
08:58

In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells

Published on: September 2, 2019

Área de la Ciencia:

  • Biología vegetal Biología vegetal
  • La ciencia molecular de las plantas.
  • Biología celular Biología celular.

Sus antecedentes:

  • La comunicación de célula a célula de las plantas se basa en el tráfico selectivo de moléculas a través de plasmodesmata.
  • Los factores de transcripción KNOTTED1 (KN1) homeobox (KNOX) son cruciales para mantener las poblaciones de células madre vegetales.

Objetivo del estudio:

  • Investigar el papel de las chaperoninas en el tráfico y la función de los factores de transcripción KNOX.
  • Para dilucidar el mecanismo por el cual las chaperoninas apoyan el mantenimiento de las células madre de las plantas.

Principales métodos:

  • Estudios de interacción genética para identificar las relaciones funcionales.
  • Los ensayos de interacción física confirman la formación de complejos proteicos.
  • Pruebas de complementación específicas del tejido para evaluar la función de las proteínas in vivo.

Principales resultados:

  • Los complejos de chaperonina son necesarios para el tráfico de célula a célula de los factores de transcripción KNOX.
  • La evidencia genética y física demuestra un vínculo funcional entre las chaperoninas y el mantenimiento de las células madre dependientes de KNOX.
  • Las chaperoninas facilitan el replegamiento de proteínas post-traducionales, esencial para el tráfico.

Conclusiones:

  • Las chaperoninas son esenciales para el tráfico de factores específicos de transcripción móvil en las plantas.
  • El tráfico de proteínas mediado por la chaperonina es crítico para el correcto funcionamiento de las células madre vegetales.