Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

Cooperative Binding of Transcription Regulators

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

DNA hypomethylating agents preserve T cell stemness and potentiate the efficacy of CD3-bispecific antibodies.

Cancer immunology research·2026

Same author

Multi-Omics and Machine Learning Integration Identifies Key Endothelial Modules in Carotid Artery Stenosis.

Journal of clinical laboratory analysis·2026

Same author

Physiologic and anesthetic predictors of false-positive motor-evoked potential alerts during pediatric spine surgery.

Spine deformity·2026

Same author

HEB collaborates with TCR signaling to upregulate <i>Id3</i> and enable γδT17 cell maturation in the fetal thymus.

eLife·2026

Same author

Targeting CRTC2 reverses <i>STK11</i> mutant NSCLC tumor resistance to immunotherapy.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Single-Cell Profiling Identifies CLEC5A+ Macrophages as Key Drivers of Thoracic Aortic Aneurysm Via CCL5-Mediated M1 Polarization.

Journal of clinical laboratory analysis·2026

Search research articles

Video Experimental Relacionado

Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

Los elementos vinculantes del CTCF median el control de la recombinación V(D) J.

Chunguang Guo¹, Hye Suk Yoon, Andrew Franklin

¹Howard Hughes Medical Institute, The Children's Hospital, The Immune Disease Institute, Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.

|September 13, 2011

Resumen

Los investigadores identificaron una nueva región reguladora, IGCR1, crucial para la recombinación de inmunoglobulina de cadena pesada (IgH) V (D) J en ratones. Esta región asegura el desarrollo adecuado de las células B y la diversidad de anticuerpos mediante el control de la reorganización del segmento genético.

Más Videos Relacionados

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

Videos de Experimentos Relacionados

Last Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

Área de la Ciencia:

Inmunología Inmunología.
Biología Molecular Biología Molecular
Genética La genética.

Sus antecedentes:

Los linfocitos B en desarrollo ensamblan exones de región variable de cadena pesada de inmunoglobulina (IgH) de los segmentos de genes V, D y J.
El locus Igh del ratón de 2,7 megabases requiere una regulación precisa de la recombinación V(D) J para repertorios de anticuerpos específicos y diversos.

Objetivo del estudio:

Identificar y caracterizar una nueva región reguladora dentro del locus Igh del ratón involucrado en la recombinación V(D) J.
Aclarar el papel funcional de esta región en el desarrollo de las células B y la formación del repertorio de anticuerpos.

Principales métodos:

Identificación de la región de control intergenico 1 (IGCR1) entre los grupos de genes V (H) y D en ratones.
Análisis funcional utilizando elementos de unión de factor de aislamiento / aislamiento CTCF y mediación de bucles Igh con potenciadores distantes.

Principales resultados:

IGCR1 inhibe la transcripción y el reordenamiento de los segmentos de genes D(H) -proximal V(H) mientras promueve el reordenamiento de los segmentos distales V(H).
IGCR1 asegura la recombinación ordenada de V, H, D, J, H, evitando las uniones incorrectas en los timocitos y regulando la retroalimentación.
IGCR1 es esencial para la regulación de la retroalimentación y la exclusión alélica de la recombinación proximal V ((H) a DJ ((H).

Conclusiones:

IGCR1 es una región reguladora crítica para la recombinación de inmunoglobulina de cadena pesada V (((D) J.
IGCR1 juega un papel clave en el desarrollo normal de las células B, el equilibrio del repertorio de anticuerpos y la recombinación específica del linaje.
El estudio destaca una función novedosa para la proteína CTCF en la regulación del locus Igh.