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La S-nitrosilación de la NADPH oxidasa regula la muerte celular en la inmunidad vegetal
Byung-Wook Yun1, Angela Feechan, Minghui Yin
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, King's Buildings, Edinburgh EH9 3JH, UK.
Nature
|October 4, 2011
Resumen
El óxido nítrico modifica los tiolos de cisteína (S-nitrosotiolos) para facilitar las respuestas inmunes de las plantas. Los altos niveles de S-nitrosotiol limitan estas respuestas inhibiendo la NADPH oxidasa, un mecanismo conservado en los eucariotas.
Área de la Ciencia:
- Inmunidad vegetal La inmunidad vegetal.
- La señalización celular de las células.
- Biología redox Biología redox.
Sus antecedentes:
- Las respuestas inmunes implican cambios en el estado redox, pero los mecanismos de señalización no están claros.
- La defensa microbiana de las plantas involucra óxido nítrico (NO) e intermediarios reactivos de oxígeno (ROI).
- El NO y el ROI están implicados en la respuesta de hipersensibilidad (HR), un mecanismo de muerte celular vegetal.
Objetivo del estudio:
- Para dilucidar los mecanismos moleculares que coordinan la síntesis de NO y ROI en la inmunidad de las plantas.
- Investigar el papel de los S-nitrosotiolos (SNOs) en la respuesta de hipersensibilidad.
- Para identificar la función de la S-nitrosilación en la regulación de la señalización inmune de las plantas.
Principales métodos:
- Análisis genético para estudiar la función del S-nitrosotiol en ausencia de ácido salicílico y ROI.
- Investigó el bucle de retroalimentación negativa que regula la respuesta hipersensible.
- Examinó la S-nitrosilación de la NADPH oxidasa (AtRBOHD) en Cys 890 y sus consecuencias funcionales.
Principales resultados:
- Los S-nitrosotiolos facilitan la respuesta de hipersensibilidad independientemente del ácido salicílico y el ROI.
- Las altas concentraciones de S-nitrosotiol desencadenan un bucle de retroalimentación negativa, lo que limita la HR.
- La S-nitrosilación de AtRBOHD en Cys 890 inhibe la síntesis de ROI, controlando la magnitud de HR.
- La mutación de Cys 890 interrumpe la regulación mediada por S-nitrosotiol de la actividad de AtRBOHD.
- La cisteína reguladora se conserva en las oxidasas NADPH humanas y en las moscas.
Conclusiones:
- Los S-nitrosotiolos son reguladores clave de las respuestas inmunes de las plantas, modulando la respuesta hipersensible.
- La S-nitrosilación de la NADPH oxidasa proporciona un mecanismo de retroalimentación negativa que controla la muerte de las células vegetales.
- Este mecanismo regulador dependiente de la S-nitrosilación se conserva en las respuestas inmunes de los animales.
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