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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Se requiere un gen candidato de proteína quinasa C, PKC1, para el ciclo celular de S. cerevisiae
D E Levin1, F O Fields, R Kunisawa
1Department of Microbiology and Immunology, University of California, San Francisco 94143.
Cell
|July 27, 1990
Resumen
La levadura en ciernes Saccharomyces cerevisiae posee un gen, PKC1, crucial para la división celular. La eliminación de este gen causa la detención del ciclo celular después de la replicación del ADN, lo que indica un nuevo punto de control del ciclo celular regulado por PKC1.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- Las isoenzimas de la proteína quinasa C (PKC) son reguladores críticos en las células de los mamíferos.
- La función de los homólogos de PKC en eucariotas inferiores como la levadura no se entiende bien.
Objetivo del estudio:
- Identificar y caracterizar genes en Saccharomyces cerevisiae relacionados con la proteína cinasa C. de los mamíferos.
- Investigar el papel del gen PKC de levadura identificado en la regulación del ciclo celular.
Principales métodos:
- Cribado del genoma de Saccharomyces cerevisiae utilizando sondas de ADNc para las isoenzimas PKC de ratas.
- Estudios de deleción y agotamiento de genes para analizar los efectos fenotípicos de la pérdida de PKC1.
- Análisis del ciclo celular de las células empobrecidas de PKC1.
Principales resultados:
- Se aisló un solo gen de levadura, PKC1, homólogo a las subespecies alfa, beta y gamma de PKC de mamíferos.
- La eliminación de PKC1 resultó en letalidad recesiva.
- Las células empobrecidas de PKC1 exhibieron un fenotipo mutante del ciclo de división celular (cdc), deteniendo la replicación posterior al ADN y antes de la mitosis con pequeños brotes, lo que sugiere un nuevo punto de control.
Conclusiones:
- El producto del gen PKC1 en Saccharomyces cerevisiae es esencial para la viabilidad celular y regula un punto de control del ciclo celular no reconocido previamente.
- Este hallazgo sugiere un papel conservado para las vías de señalización de PKC en el control del ciclo celular a través de eucariotas.
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