Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

11.0K
Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker...
11.0K
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

6.9K
The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC...
6.9K
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

24.0K
Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced...
24.0K
In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors12:12

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

13.0K
This study demonstrates the reprogramming of somatic cells towards pluripotency in vivo without the generation of teratomas. We used hydrodynamic tail vein injection of plasmid DNA encoding the Yamanka factors to induce the in vivo reprogramming of adult hepatocytes into cells of enhanced...
13.0K
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set12:10

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

30.6K
We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific...
30.6K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.6K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.6K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Modeling immune responses to autologous and allogeneic human stem cell-derived islet grafts in vivo.

JCI insight·2026
Same author

Asymmetric attrition and secondary chromosome destabilization after double-strand breaks in human embryonic development.

Nature communications·2026
Same author

Genetics of growth rate in induced pluripotent stem cells.

Stem cell reports·2026
Same author

National dialysis registry of the Dominican Republic: first nationwide epidemiological analysis of renal replacement therapy.

BMC nephrology·2026
Same author

Patient induced pluripotent stem cells identify specificities of a reticular pseudodrusen phenotype in age-related macular degeneration.

Genome medicine·2026
Same author

A loss of function variant in <i>SLC30A8/ZnT8</i> drives proteomic changes associated with lowered apoptosis in human stem cell-derived islets.

medRxiv : the preprint server for health sciences·2026

Video Experimental Relacionado

Updated: Jan 20, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

6.9K

Los ovocitos humanos reprograman las células somáticas a un estado pluripotente.

Scott Noggle1, Ho-Lim Fung, Athurva Gore

  • 1The New York Stem Cell Foundation Laboratory, New York, New York, USA.

Nature
|October 8, 2011
PubMed
Resumen

El intercambio de genoma en ovocitos humanos para el modelado de enfermedades falló debido a una detención del desarrollo. Sin embargo, la adición de un genoma somático a un ovocito intacto permitió el desarrollo del blastocisto y la derivación de células madre pluripotentes.

Más Videos Relacionados

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.0K
In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
12:12

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

Published on: December 17, 2013

13.0K

Videos de Experimentos Relacionados

Last Updated: Jan 20, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

6.9K
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.0K
In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
12:12

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

Published on: December 17, 2013

13.0K

Área de la Ciencia:

  • Biología de la reproducción Biología reproductiva.
  • La ciencia de las células madre.
  • Genética humana Genética humana es la genética humana.

Sus antecedentes:

  • La terapia de reemplazo celular es prometedora para el tratamiento de enfermedades humanas degenerativas.
  • La generación de células específicas del paciente requiere la reprogramación de las células somáticas en células madre pluripotentes.

Objetivo del estudio:

  • Investigar la viabilidad de generar células madre pluripotentes específicas del paciente a través del intercambio del genoma de ovocitos humanos.
  • Identificar los factores que limitan la reprogramación y el desarrollo exitosos.

Principales métodos:

  • Los ovocitos humanos se sometieron a un intercambio de genoma con núcleos de células somáticas.
  • Alternativamente, se agregaron núcleos de células somáticas a los ovocitos intactos, creando células triploides.
  • Las células derivadas se evaluaron para el potencial de desarrollo y los marcadores de pluripotencia.

Principales resultados:

  • El intercambio de genomas condujo a la detención del desarrollo en las últimas etapas de la escisión con anomalías transcripcionales.
  • La adición de genomas somáticos a los ovocitos intactos resultó en el desarrollo de blastocistos.
  • Las líneas de células madre derivadas de estos blastocistos exhibieron pluripotencia y se diferenciaron en las tres capas germinales.

Conclusiones:

  • La eliminación del genoma del ovocito es la causa principal de la insuficiencia del desarrollo en los ovocitos intercambiados por genoma.
  • La reprogramación de las células somáticas humanas utilizando ovocitos es factible cuando se conserva el genoma del ovocito.
  • Este enfoque permite la generación de células madre pluripotentes específicas del paciente para el modelado de enfermedades y posibles terapias.