Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Nucleic acids

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Nucleic Acids

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Nucleic Acid Structure

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Distinct RNA engagements define genome import and replication elongation in alphaviruses.

bioRxiv : the preprint server for biology·2026

Same author

A self-amplifying RNA vector based on rubella virus for mRNA therapeutics and vaccine applications.

Molecular therapy. Nucleic acids·2026

Same author

DDX6 induces immunosuppression in cancer by disrupting structural stability of endogenous double-stranded RNAs.

Science immunology·2026

Same author

Sequence alignment of the primate lineage reveals evolutionary divergence and conserved secondary structural motifs in noncoding RNAs.

bioRxiv : the preprint server for biology·2026

Same author

Conserved structural features of the lncRNA HOTAIR in breast cancer cells.

bioRxiv : the preprint server for biology·2026

Same author

Pnky lncRNA secondary structure maps identify functional regions that control neurogenesis in neural stem cells.

Cell reports·2026

Search research articles

Video Experimental Relacionado

Updated: May 13, 2026

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

Conocimientos estructurales sobre el reconocimiento de ARN por RIG-I.

Dahai Luo¹, Steve C Ding, Adriana Vela

¹Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.

|October 18, 2011

Resumen

Los investigadores revelaron la estructura de RIG-I, una proteína clave en la inmunidad antiviral, unida al ARN viral. Esta estructura aclara cómo RIG-I reconoce patógenos e inicia respuestas inmunes.

Más Videos Relacionados

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Videos de Experimentos Relacionados

Last Updated: May 13, 2026

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE

Published on: July 29, 2014

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

Área de la Ciencia:

Biología Estructural Biología estructural.
Inmunología Inmunología.
Biología Molecular Biología Molecular

Sus antecedentes:

Los receptores intracelulares tipo RIG-I (RLR) detectan el ARN viral, iniciando la inmunidad antiviral.
Comprender los mecanismos moleculares de las RLR es crucial para la investigación de la defensa del huésped.

Objetivo del estudio:

Para dilucidar la base molecular del reconocimiento y activación del ARN de RIG-I.
Para determinar la estructura cristalina de RIG-I en complejo con ARN de doble cadena (dsRNA).

Principales métodos:

Se utilizó cristalografía de rayos X para determinar la estructura de RIG-I unido al dsRNA.
Se realizó un análisis bioinformático para comprender las interacciones del dominio de la proteína.

Principales resultados:

El dsRNA está encerrado por dominios RIG-I: helicasa (HEL1, HEL2), inserción HEL2 (HEL2i) y dominio regulador C-terminal (CTD).
Una pinza en forma de V enlaza HEL2 y CTD, coordinando las funciones de dominio y acoplando la unión de ARN a la hidrólisis de ATP.
RIG-I, una helicasa de la superfamilia 2, exhibe una interacción compleja entre los dominios motores, los dominios accesorios y el ARN.

Conclusiones:

La estructura determinada proporciona información sobre la función nanomecánica de RIG-I en la inmunidad antiviral.
Este trabajo tiene implicaciones más amplias para la comprensión de la función de las ATPasas y las helicasas.