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Interconversión entre poblaciones de células madre intestinales en nichos distintos.

Norifumi Takeda¹, Rajan Jain, Matthew R LeBoeuf

¹Department of Cell and Developmental Biology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19104, USA.

Science (New York, N.Y.)

|November 15, 2011

Resumen

Los investigadores identificaron Hopx como un marcador para las células madre intestinales +4. Estas células +4 pueden generar células columnares de base criptográfica (CBC), y las CBC pueden generar células +4, revelando una relación bidireccional entre los tipos de células madre.

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Área de la Ciencia:

Gastroenterología y Gastroenterología.
Biología de las células madre Biología de las células madre
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

Las células madre epiteliales intestinales son cruciales para la homeostasis de los tejidos.
Dos poblaciones distintas de células madre, las células +4 y las células columnares de base criptográfica (CBC), residen en nichos diferentes.
La relación entre +4 y las células CBC sigue siendo poco comprendida.

Objetivo del estudio:

Para aclarar la relación entre las células madre intestinales +4 y CBC.
Para identificar marcadores específicos para distintas poblaciones de células madre intestinales.

Principales métodos:

Utilizado Hopx como un marcador específico para las células +4.
Investigó el rastreo del linaje y la determinación del destino celular en la cripta intestinal.

Principales resultados:

Hopx es un marcador específico para la población quiescente de células madre intestinales +4.
+4 Las células que expresan Hopx pueden diferenciarse en células CBC y todos los demás linajes epiteliales intestinales.
Las células columnares de base de cripta (CBC, por sus siglas en inglés) también pueden diferenciarse en células +4 Hopx positivas.

Conclusiones:

Existe una relación de linaje bidireccional entre las células madre intestinales activas (CBC) y quiescentes (+4).
Esta relación bidireccional pone de relieve una jerarquía dinámica de células madre en el epitelio intestinal.