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Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
Published on: May 1, 2020
Una toxina de Burkholderia pseudomallei inhibe la actividad de la helicasa del factor de traducción eIF4A
Abimael Cruz-Migoni1, Guillaume M Hautbergue, Peter J Artymiuk
1Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK.
Science (New York, N.Y.)
|November 15, 2011
Resumen
Una proteína recién descubierta, BPSL1549, de Burkholderia pseudomallei es una potente citotoxina letal para los ratones. Inhibe la traducción de proteínas al desamidar el factor de iniciación de la traducción eIF4A.
Área de la Ciencia:
- Microbiología Microbiología.
- Toxicología Toxicología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Burkholderia pseudomallei es un patógeno oportunista.
- La función de BPSL1549, una proteína de B. pseudomallei, era previamente desconocida.
- Los factores necrotizantes citotóxicos son factores de virulencia conocidos en otras bacterias.
Objetivo del estudio:
- Para caracterizar la función y estructura de BPSL1549.9.
- Para investigar el mecanismo de toxicidad de BPSL1549.
- Proponer un nombre para BPSL1549 basado en su función.
Principales métodos:
- Análisis estructural del BPSL1549.
- En ensayos de citotoxicidad in vitro en células eucariotas.
- Estudios de letalidad in vivo en ratones.
- Análisis bioquímicos para determinar el objetivo de BPSL1549.
Principales resultados:
- BPSL1549 comparte similitud estructural con el citotóxico factor necrotizante 1 de E. coli.
- BPSL1549 es una potente citotoxina y es letal para los ratones.
- BPSL1549 desamidata la glutamina-339 del factor de iniciación de la traducción eIF4A, inhibiendo su actividad de helicasa y bloqueando la traducción.
- La expresión de bpsl1549 se correlaciona con la patogenicidad.
Conclusiones:
- BPSL1549 es una nueva toxina bacteriana.
- BPSL1549 funciona inhibiendo la síntesis de proteínas a través de la modificación de eIF4A.
- Proponemos darle el nombre de BPSL1549 Burkholderia factor letal 1 (BLF1).
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