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Una red de genes HLH ampliamente expresados regula el destino celular específico del tejido
Abhishek Bhattacharya1, Nicholas E Baker
1Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
Cell
|November 15, 2011
Resumen
Una red reguladora que involucra a las proteínas Daughterless (Da) y Extramacrochaetae (Emc) controla la diferenciación celular. Este mecanismo conservado equilibra la diferenciación con el crecimiento y la supervivencia, impactando la regulación del desarrollo por las proteínas hélice-bucle-hélice.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los factores de transcripción de hélice básica, bucle y hélice (bHLH) son cruciales para la diferenciación celular.
- La expresión de los socios heterodímeros bHLH influye significativamente en los resultados de la diferenciación.
- Comprender la regulación de estos socios es clave para comprender los procesos de desarrollo.
Objetivo del estudio:
- Investigar un mecanismo regulador distinto que controle la expresión de los socios heterodímeros de bHLH.
- Aclarar el papel de las proteínas Daughterless (Da) y Extramacrochaetae (Emc) en el desarrollo de Drosophila.
- Para determinar si este mecanismo regulador se conserva en diferentes tejidos y especies.
Principales métodos:
- Análisis de una red reguladora de interacción cruzada en Drosophila.
- Investigar los circuitos de retroalimentación transcripcional que involucran a Emc y Da.
- Examinando el impacto de las señales extracelulares en la expresión de Emc.
- Análisis comparativo en múltiples tejidos de Drosophila y células de mamíferos.
Principales resultados:
- Una red reguladora enlaza la expresión de Da y Emc a través de bucles de retroalimentación.
- La expresión generalizada de Emc restringe Da, oponiéndose a la diferenciación mientras promueve el crecimiento y la supervivencia.
- Las señales extracelulares que reprimen Emc conducen a un aumento de la expresión de Da.
- Esta regulación define las regiones ectodérmicas proneurales independientemente de los genes bHLH.
Conclusiones:
- Un mecanismo regulador conservado que involucra a Da y Emc equilibra la diferenciación con el crecimiento y la supervivencia.
- Esta red proporciona un mecanismo para definir los territorios de desarrollo.
- Los hallazgos sugieren una característica general de la regulación conservada del desarrollo por las proteínas hélice-bucle-hélice.
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