Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Cross-reactivity00:42

Cross-reactivity

Overview
Transduction01:16

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
Dose-Response Relationship: Selectivity and Specificity01:25

Dose-Response Relationship: Selectivity and Specificity

Drugs exert their therapeutic effects by interacting with receptors, enzymes, or ion channels that are present throughout the human body. The strength and duration of the interaction between a drug and its target receptor are characterized by the selectivity and specificity of the drug. Selectivity refers to a drug's strong preference for its intended target over other targets. For instance, isoprenaline, a non-selective β-adrenergic agonist, interacts with both β1- and β2-adrenergic receptors...
Evolutionary Psychology01:20

Evolutionary Psychology

Evolutionary psychology explores the origins of human behavior and mental processes by framing them within the context of natural selection, a theory famously propounded by Charles Darwin. This field asserts that many behaviors common across human societies — ranging from instinctive fear reactions to complex social interactions — arose as evolutionary adaptations. These adaptations enhanced the survival and reproductive success of our ancestors, thereby becoming embedded in the human psyche...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Evolution of New Traits in Microbes01:24

Evolution of New Traits in Microbes

Microorganisms evolve rapidly due to their large population sizes and short generation times, often exhibiting measurable changes within days under laboratory conditions. Natural selection acts on standing genetic variation, enabling the retention and amplification of beneficial traits that confer fitness advantages in changing environments.Adaptive Pigment Regulation in RhodobacterIn Rhodobacter, a genus of purple non-sulfur bacteria, light-harvesting pigments such as bacteriochlorophyll and...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Injectable Thermosensitive Placental ECM-Copper Hydrogel With Endothelial Progenitor Cells for Pressure Ulcer Repair.

Journal of biomedical materials research. Part B, Applied biomaterials·2026
Same author

Advanced extracellular vesicle therapeutics: From molecular engineering to intelligent devices.

Acta biomaterialia·2026
Same author

Biomimetic hydrogels integrated with strontium and zinc co-substituted mesoporous silica nanoparticles with osteo-immunomodulatory and osteo-angiogenic functions for advanced bone regeneration.

Biomaterials advances·2026
Same author

3D-printed functional engineered intestinal models.

Journal of materials chemistry. B·2026
Same author

Dynamic Targetable Extracellular Vesicle Surface Proteins Monitor Depth of Response to CAR T Therapy.

Research square·2026
Same author

PID-controller enhanced artificial β-cells.

PloS one·2026

Video Experimental Relacionado

Updated: May 26, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

Optimización de la reactividad cruzada con búsqueda evolutiva de sensores.

Kyung-Ae Yang1, Renjun Pei, Darko Stefanovic

  • 1Division of Experimental Therapeutics, Department of Medicine, Columbia University, New York, New York 10032, USA.

Journal of the American Chemical Society
|December 7, 2011
PubMed
Resumen

Los investigadores desarrollaron un método evolutivo para crear matrices de sensores de ADN para clasificar con precisión los esteroides. Este enfoque permite una discriminación precisa entre las diferentes clases de esteroides, ofreciendo una nueva herramienta de diagnóstico.

Más Videos Relacionados

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
09:39

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Videos de Experimentos Relacionados

Last Updated: May 26, 2026

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow
08:58

Efficient Sampling of Genetically Encoded Biosensor Design Space Enabled with a Design of Experiments and Automation Workflow

Published on: October 17, 2025

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications
09:39

Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications

Published on: January 27, 2014

Área de la Ciencia:

  • Biotecnología La biotecnología es la biotecnología.
  • Biología Molecular Biología Molecular
  • La genómica es la genómica.

Sus antecedentes:

  • El desarrollo de biosensores selectivos es crucial para el diagnóstico molecular.
  • Los sensores basados en ADN ofrecen capacidades de interacción únicas.
  • La clasificación de mezclas moleculares complejas como los esteroides requiere una alta especificidad.

Objetivo del estudio:

  • Diseñar un procedimiento evolutivo sencillo para construir una matriz óptima de sensores de ADN.
  • Para lograr una clasificación perfecta de los esteroides individuales utilizando una matriz de sensores.
  • Para demostrar la capacidad discriminatoria entre las clases de esteroides a través de rangos de concentración.

Principales métodos:

  • Secuencias de ADN de minería orientadas hacia uniones de tres vías desde un grupo más grande.
  • Aplicación de presiones de selección separadas a los sensores individuales para la interacción de esteroides.
  • Permitir una reactividad cruzada controlada para mejorar la precisión de la clasificación.
  • Montando una matriz de tres sensores para el análisis de esteroides.

Principales resultados:

  • Se construyó con éxito una matriz óptima de sensores utilizando el procedimiento evolutivo.
  • La matriz de sensores demostró la capacidad discriminatoria entre las clases de esteroides.
  • Se logró una clasificación precisa de los esteroides individuales a través de los rangos de concentración completa.
  • La matriz estaba compuesta por tres sensores de ADN distintos.

Conclusiones:

  • El procedimiento evolutivo reportado es efectivo para construir conjuntos óptimos de sensores de ADN.
  • Este método se puede aplicar para clasificar muestras con distintas proporciones de componentes.
  • La matriz de sensores desarrollada ofrece una herramienta robusta para el análisis de esteroides y potencialmente otras tareas de clasificación molecular.