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Videos de Conceptos Relacionados

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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Video Experimental Relacionado

Updated: May 26, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

La unión del cofactor evoca diferencias latentes en la especificidad de unión al ADN entre las proteínas Hox.

Matthew Slattery1, Todd Riley, Peng Liu

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168(th) Street, HHSC 1104, New York, NY 10032, USA.

Cell
|December 14, 2011
PubMed
Resumen
Este resumen es generado por máquina.

Los factores de transcripción adquieren nuevas habilidades de unión al ADN cuando forman complejos con cofactores. Este estudio revela cómo las proteínas de Drosophila Hox cambian las propiedades de reconocimiento con el cofactor Extradenticle-Homothorax.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética La genética.
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • Las familias de factores de transcripción comparten similitudes de unión al ADN, pero tienen distintas funciones in vivo.
  • Los factores de transcripción a menudo se unen al ADN como complejos multiproteicos, lo que sugiere especificidades de unión al ADN alteradas.

Objetivo del estudio:

  • Para investigar si la formación de complejos de factores de transcripción modifica las especificidades de unión al ADN.
  • Desarrollar un método para determinar las afinidades de secuencias de ADN de complejos de factores de transcripción.

Principales métodos:

  • Desarrolló SELEX-seq, una plataforma experimental y computacional.
  • Se aplicó SELEX-seq a las ocho proteínas de Drosophila Hox y al cofactor Extradenticle-Homothorax (Exd).

Principales resultados:

  • Las proteínas de Drosophila Hox exhiben nuevas propiedades de reconocimiento de ADN cuando se complejan con Exd.
  • Las especificidades de Exd-Hox se dividen en tres clases, alineadas con la colinealidad del gen Hox.
  • Las predicciones de la estructura del ADN indican distintas preferencias de topografía de ranura menor para las proteínas Hox anterior y posterior.

Conclusiones:

  • Las interacciones de cofactores, como Exd con proteínas Hox, conducen a propiedades emergentes de reconocimiento de ADN.
  • Estas propiedades emergentes contribuyen a la especificidad in vivo de los factores de transcripción.