Jove
Visualize
Contáctanos

Videos de Conceptos Relacionados

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

RNA imprinting by zinc-finger proteins.

Trends in biochemical sciences·2026
Same author

Cannabidiol polarizes human neutrophils toward a cancer-promoting phenotype.

Frontiers in immunology·2025
Same author

Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus.

PloS one·2025
Same author

The zinc-finger transcription factor Sfp1 imprints specific classes of mRNAs and links their synthesis to cytoplasmic decay.

eLife·2024
Same author

Comparison of Xrn1 and Rat1 5' → 3' exoribonucleases in budding yeast supports the specific role of Xrn1 in cotranslational mRNA decay.

Yeast (Chichester, England)·2024
Same author

RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway.

Nature communications·2022
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Ver todos los artículos relacionados
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Video Experimental Relacionado

Updated: May 11, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Los elementos promotores regulan la desintegración del ARNm en el citoplasma.

Almog Bregman1, Moran Avraham-Kelbert, Oren Barkai

  • 1Department of Molecular Microbiology, Technion-Israel Institute of Technology, Haifa 31096, Israel.

Cell
|December 27, 2011
PubMed
Resumen
Este resumen es generado por máquina.

Los promotores de la levadura, específicamente la secuencia de activación aguas arriba (UAS) y la proteína Rap1p, pueden controlar las tasas de desintegración del ARN mensajero (ARNm) después de la transcripción. Este hallazgo revela un papel novedoso para los promotores en la regulación de la expresión génica post-transcripcionalmente.

Más Videos Relacionados

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Videos de Experimentos Relacionados

Last Updated: May 11, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
10:56

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Published on: May 17, 2014

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells
14:29

Rapid In Vivo Fixation and Isolation of Translational Complexes from Eukaryotic Cells

Published on: December 25, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Genética de la levadura Genética de la levadura
  • Reglamento genético Reglamento genético.

Sus antecedentes:

  • Los promotores son secuencias de ADN cruciales para iniciar la transcripción y están regulados por factores de acción trans.
  • La comprensión convencional de los promotores se limita a su papel en la síntesis génica, no en la regulación post-transcripcional.

Objetivo del estudio:

  • Para investigar si los promotores de levadura influyen en las tasas de desintegración del ARNm después de la transcripción.
  • Identificar elementos promotores específicos y factores involucrados en la regulación de la estabilidad del ARNm.

Principales métodos:

  • Utilizó un sistema de genes reporteros en la levadura para evaluar la cinética de desintegración del ARNm.
  • Secuencias de activación aguas arriba manipuladas (UAS) del gen reportero.
  • Investigó el papel de los sitios de unión a Rap1p y la proteína Rap1p en la descomposición del ARNm.

Principales resultados:

  • La alteración de las secuencias UAS de un gen reportero, sin cambiar la transcripción, modificó su tasa de descomposición.
  • Un elemento cis específico con dos sitios de unión a Rap1p, junto con Rap1p, fue suficiente para mejorar la descomposición del ARNm.
  • Se encontró que Rap1p estimula tanto la síntesis como la descomposición de ciertos ARNm endógenos.

Conclusiones:

  • Los promotores de la levadura, a través de elementos como UAS y proteínas como Rap1p, pueden regular la descomposición del ARNm en el citoplasma.
  • Los factores vinculados al promotor pueden influir en la composición de la ribonucleoproteína mensajera (mRNP), afectando la estabilidad del ARNm.
  • Los promotores juegan un doble papel en la coordinación de la síntesis y la descomposición del ARNm, lo que afecta a los niveles generales de ARNm.