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Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
Published on: December 10, 2015
Función conservada de los lincRNA en el desarrollo embrionario de los vertebrados a pesar de la rápida evolución de
Igor Ulitsky1, Alena Shkumatava, Calvin H Jan
1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Cell
|December 27, 2011
Resumen
Los investigadores identificaron más de 550 ARN no codificantes de larga duración (ARN linc) en el pez cebra. Estos lincRNA juegan un papel vital en el desarrollo embrionario, con funciones conservadas a pesar de la mínima similitud de secuencia entre especies.
Área de la Ciencia:
- La genómica es la genómica.
- Biología del desarrollo Biología del desarrollo.
- ARN Biología Biología ARN
Sus antecedentes:
- Se han identificado en los mamíferos miles de ARN no codificantes de larga duración (ARN linc).
- Comprender la evolución y la función de estos ARN es crucial.
Objetivo del estudio:
- Para identificar y caracterizar los lincRNA en el pez cebra.
- Investigar la conservación evolutiva y las funciones funcionales de los lincRNAs del pez cebra durante el desarrollo embrionario.
Principales métodos:
- Utilizó marcas de cromatina, mapeo del sitio poli (A) y datos de RNA-Seq para la identificación de lincRNA.
- Empleó reactivos antisense para atacar regiones conservadas y sitios de empalme de lincRNAs de pez cebra.
- Evalúa los defectos del desarrollo y los experimentos de rescate utilizando lincRNAs maduros y sus ortólogos de mamíferos.
Principales resultados:
- Identificó más de 550 lincRNAs distintos en el pez cebra, compartiendo características con los lincRNAs de los mamíferos.
- Se encontró una similitud de secuencia limitada (29/550) con ortólogos de mamíferos, a menudo restringidos a regiones conservadas.
- Se demostró que la orientación a regiones conservadas o sitios de empalme de lincRNAs de pez cebra causó defectos de desarrollo, que fueron rescatados por lincRNAs homólogos.
- Mostró ubicaciones genómicas conservadas para algunos lincRNA sin conservación de secuencia.
Conclusiones:
- Los lincRNAs del pez cebra juegan un papel crítico en el desarrollo embrionario.
- La funcionalidad del lincRNA se conserva en todas las especies a pesar de la conservación limitada de la secuencia.
- Este estudio proporciona un marco para la identificación y el análisis de lincRNA en organismos modelo.
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