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Un papel para la integrina en la formación de la citoarquitectura sarcomérica
T Volk1, L I Fessler, J H Fessler
1Molecular Biology Institute, University of California, Los Angeles 90024-1606.
Cell
|November 2, 1990
Resumen
Las integrinas son cruciales para el desarrollo muscular, coordinando la estructura interna con el medio ambiente. Las mutaciones en las integrinas interrumpen la formación de sarcómeros y la integración muscular en Drosophila.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las integrinas son receptores de la superficie celular que median las interacciones de la matriz celular-extracelular.
- La diferenciación muscular implica una compleja organización citoarquitectónica y la integración de los tejidos.
- El papel de las integrinas en la coordinación de la citoarquitectura muscular y la integración de los tejidos requiere una mayor aclaración.
Objetivo del estudio:
- Investigar el papel de las integrinas en la diferenciación muscular y la formación de sarcómeros.
- Determinar la necesidad de integrinas para la correcta integración de las células musculares en el tejido.
- Examinar la localización y función de las integrinas durante la miogénesis en Drosophila.
Principales métodos:
- Cultivo de células embrionarias de Drosophila en un sustrato de laminina.
- Análisis de la localización de la integrina mediante inmunofluorescencia.
- Comparación de embriones/larvas de tipo salvaje y integrina beta PS mutante nulo (miosferoide).
- Evaluación de la miogénesis y la formación de sarcomeros.
Principales resultados:
- Integrin alfa PS2 beta PS localizado a las regiones de contacto en las células cultivadas y a las bandas Z y las inserciones musculares in vivo.
- Se produjo la formación de miotubos, pero el desarrollo del sarcoma requirió suero o fibronectina.
- La deficiencia de integrina en los mutantes miosferoides condujo a una miogénesis defectuosa y a la formación de la banda Z.
- La adhesión celular, la propagación y el crecimiento en la laminina fueron independientes de la integrina.
Conclusiones:
- Las integrinas son esenciales para coordinar la citoarquitectura del sarcomero con el entorno celular durante la diferenciación muscular.
- Las integrinas juegan un papel crítico en la correcta integración de las células musculares en el desarrollo de los tejidos.
- Mientras que las integrinas son vitales para la organización del sarcomero, otras proteínas median en la fijación inicial del mioblasto y la neurona a la laminina.
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