Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Dynamics of an RNase H-Responsive Tetrahedral DNA Nanostructure for Efficient Intracellular microRNA Inhibition.

Bioconjugate chemistry·2026
Same author

How I do it: Zone 0 arch treatment with laser in situ fenestrated endograft repair.

Journal of vascular surgery cases and innovative techniques·2026
Same author

Exploring Size-Controlled Exciton Evolution Using DNA Libraries.

Journal of the American Chemical Society·2026
Same author

Thoracoabdominal multibranch endograft repair of type Ia endoleak after laser in situ fenestration and chimney endovascular repair for pararenal aortic aneurysm.

Journal of vascular surgery cases and innovative techniques·2026
Same author

NIR-Actuated Morphodynamic 2D Nanopatches for Interface-Programmed Immunoactivation and Tumor Regression.

Journal of the American Chemical Society·2026
Same author

Polymerisation processes and computational methods to control structure: general discussion.

Faraday discussions·2025
Same journal

Proton-Gated Torsional Spring for Molecular Energy Storage.

Journal of the American Chemical Society·2026
Same journal

Topologically Programmed Dual-Channel Covalent Organic Frameworks Decouple Gas and Ion Fluxes for Acidic CO<sub>2</sub> Electroreduction.

Journal of the American Chemical Society·2026
Same journal

Plasmonic Re-Excitation Enables Superoxide-Mediated Ethane Conversion to Acetic Acid under Visible Light.

Journal of the American Chemical Society·2026
Same journal

Photocatalytic Controlled Halodefluorination of Perfluoroalkyl Compounds Using <i>N</i>-Arylphenothiazines.

Journal of the American Chemical Society·2026
Same journal

Photoinduced Disproportionation Enables Oxidative Addition of Aryl Iodides at a Gallium(I) Center.

Journal of the American Chemical Society·2026
Same journal

Biocatalytic C3 β-<i>O</i>-Glycosylation of Triterpenes and Sterols to Synthesize Natural and Unnatural Saponins.

Journal of the American Chemical Society·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Síntesis orgánica programable de una sola olla en varios pasos utilizando uniones de ADN.

Mireya L McKee1, Phillip J Milnes, Jonathan Bath

  • 1Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU, UK.

Journal of the American Chemical Society
|January 27, 2012
PubMed
Resumen
Este resumen es generado por máquina.

Este estudio introduce un nuevo sistema de síntesis con plantilla de ADN para la producción controlada de oligómeros. El método permite una síntesis eficiente y paralela en una sola olla, reduciendo los pasos de reacción para moléculas complejas.

Más Videos Relacionados

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Videos de Experimentos Relacionados

Last Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Área de la Ciencia:

  • La bioquímica es la bioquímica.
  • Biología Molecular Biología Molecular
  • Química sintética de la química sintética.

Sus antecedentes:

  • La síntesis en múltiples etapas de oligómeros es compleja y a menudo requiere múltiples pasos de reacción y etapas de purificación.
  • Los métodos actuales pueden verse limitados por las condiciones de reacción que cambian con la longitud del oligómero.
  • Las estrategias de síntesis y ligadura paralelas pueden reducir el tiempo de síntesis general, pero requieren un control cuidadoso.

Objetivo del estudio:

  • Desarrollar un nuevo sistema para la síntesis de ADN en múltiples etapas.
  • Para permitir la síntesis de oligómeros controlada por secuencia en un entorno de reacción constante.
  • Para facilitar la síntesis paralela y la ligadura dentro de un único recipiente de reacción.

Principales métodos:

  • Utilizando la formación secuencial de las uniones de ADN para controlar el proceso de síntesis.
  • La fijación de reactivos a los adaptadores de ADN que se hibridan a las hebras de la plantilla de ADN.
  • Repitiendo los pasos de hibridación y reacción para la síntesis iterativa.
  • Realizar la síntesis en una sola olla con todos los monómeros reactivos necesarios.
  • Ligando productos de síntesis paralela para reducir los pasos generales.

Principales resultados:

  • Demostró un sistema para la síntesis controlada de ADN en varios pasos.
  • Se logró la síntesis de oligómeros controlada por secuencia con un entorno de reacción constante.
  • Permitió la síntesis paralela de diferentes oligómeros en el mismo recipiente.
  • Se mostró la ligación de productos de síntesis paralelas para reducir los pasos.

Conclusiones:

  • El sistema desarrollado con plantilla de ADN ofrece una plataforma robusta para la síntesis eficiente de oligómeros.
  • El método permite el control de secuencia y el procesamiento en paralelo en un solo pot.
  • Este enfoque simplifica la síntesis compleja, reduce los pasos de reacción y mejora la productividad.