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Following Cell-fate in E. coli After Infection by Phage Lambda

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Published on: October 14, 2011

Repetibilidad y contingencia en la evolución de una innovación clave en el fago lambda.

Justin R Meyer¹, Devin T Dobias, Joshua S Weitz

¹Department of Zoology, Michigan State University, East Lansing, MI 48824, USA. justin.raymond.meyer@gmail.com

Science (New York, N.Y.)

|January 28, 2012

Resumen

Las innovaciones evolutivas surgen de una compleja interacción entre los cambios genómicos virales y las condiciones ecológicas del huésped. El bacteriófago lambda desarrolló nuevas vías de infección al adaptar su proteína de reconocimiento de huésped, lo que demuestra cómo surgen nuevas funciones.

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Área de la Ciencia:

Biología evolutiva Biología evolutiva.
Virología Virología.
La genómica es la genómica.

Sus antecedentes:

La evolución de las innovaciones clave, que otorgan a los linajes nuevas funciones y oportunidades ecológicas, no se entiende bien.
Comprender los mecanismos detrás de la innovación evolutiva es crucial para varias disciplinas biológicas.

Objetivo del estudio:

Para investigar la vía evolutiva del bacteriófago lambda que infecta a su huésped, Escherichia coli, a través de un nuevo mecanismo.
Aclarar la interacción entre las mutaciones genómicas virales y los factores ecológicos del huésped en la aparición de innovaciones evolutivas.

Principales métodos:

Se examinaron mutaciones en la proteína de reconocimiento de huésped J. del bacteriófago lambda.
Cambios analizados en la expresión del receptor del huésped (Escherichia coli) (LamB y OmpF).
Investigó el impacto de las mutaciones genómicas del huésped en la adaptación viral.

Principales resultados:

La selección natural favoreció las mutaciones en la proteína J, mejorando la aptitud en el receptor original (LamB).
Estas mutaciones facilitaron las adaptaciones posteriores para la infección a través de un nuevo receptor (OmpF).
La evolución del huésped (expresión LamB reducida) y las mutaciones específicas del huésped influyeron en la capacidad del fago para adquirir nuevas funciones.

Conclusiones:

La evolución viral de las innovaciones clave está impulsada por una combinación de procesos genómicos y presiones ecológicas.
La adaptación del bacteriófago lambda demuestra una nueva vía para adquirir nuevas funciones, destacando la coevolución dinámica entre virus y huéspedes.
Este estudio subraya la intrincada relación entre los cambios genéticos y las condiciones ambientales en la configuración de la innovación evolutiva.