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Tao Li¹, Damian Ackermann, Anna M Hall

¹Life and Medical Science Institute, Program Unit Chemical Biology and Medicinal Chemistry, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.

Journal of the American Chemical Society

|February 3, 2012

Resumen

Los cambios en el potasio y el pH desencadenan conversiones estructurales de ácido nucleico, lo que permite operaciones de lógica molecular. Estas estructuras de ADN y ARN, cuando se combinan con la hemina, actúan como reporteros para la fluorescencia o la detección colorimétrica de puertas lógicas.

Área de la Ciencia:

Bioquímica y Biología Molecular.
Química supramolecular de las moléculas.
Nanotecnología La nanotecnología es la nanotecnología.

Sus antecedentes:

Los ácidos nucleicos exhiben diversas formas estructurales más allá de los dúplex canónicos, incluidos los G-cuadruplexos y los i-motivos.
Estas estructuras son sensibles a los iones (por ejemplo, K +) y el pH, lo que lleva a cambios de conformación.
Tales transiciones estructurales que responden a estímulos ofrecen potencial para el procesamiento de información molecular.

Objetivo del estudio:

Para investigar las conversiones estructurales desencadenadas por K(+) - y H(+) de varias hélices de ácido nucleico.
Para explorar la aplicación de estas interconversiones estructurales en la realización de operaciones de lógica molecular.
Establecer un mecanismo de señalización para la detección de estos eventos moleculares utilizando complejos G-cuadruplex/hemina.

Principales métodos:

La electroforesis en gel, el dicroísmo circular y la desnaturalización térmica se utilizaron para estudiar las conversiones estructurales.
Se emplearon fluorimetría y colorimetría para verificar las operaciones de lógica molecular.
Hibridación de hebras ricas en G y ricas en C, seguidas de estímulos de K+ y pH, y formación de complejo G-cuadruplex/hemina para la detección de señales.

Principales resultados:

La adición de K+ indujo el desenrollo de duplexos y el plegamiento de hebras ricas en G en G-cuadruplexos.
La disminución del pH condujo a la formación de i-motif en el ADN o la formación de triplex en el ARN, dependiendo de la composición de las hebras.
Los complejos G-cuadruplex/hemina catalizan reacciones que producen señales fluorescentes o colorimétricas, permitiendo operaciones de puerta lógica (NOR, INH, AND).

Conclusiones:

Las interconversiones estructurales de ácido nucleico impulsadas por K+ y pH pueden aprovecharse para el cálculo molecular.
El sistema G-quadruplex/hemin proporciona una plataforma versátil para la transducción de señales en puertas de lógica molecular.
Este estudio demuestra un marco para el desarrollo de dispositivos moleculares basados en ácido nucleico receptivos.

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