Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

Long-patch Base Excision Repair

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Fixing Double-strand Breaks

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

Cryo-EM structure determination of amyloid fibrils: Methodology, insights, and practical tools.

Methods in enzymology·2026

Same author

Stochastic misfolding drives the emergence of distinct α-synuclein strains.

Neuron·2026

Same author

Two-photon excitation enables single-molecule detection of a fluorescent base analogue in DNA with high photostability.

Chemical science·2026

Same author

Cryo-EM studies of amyloid-β fibrils from human and murine brains carrying the Uppsala APP mutation (Δ690-695).

Acta neuropathologica communications·2025

Same author

Anle138b binds predominantly to the central cavity in lipidic Aβ₄₀ fibrils and modulates fibril formation.

Nature communications·2025

Same author

Resolution of physics and deep learning-based protein engineering filters: A case study with a lipase for industrial substrate hydrolysis.

PloS one·2025

Search research articles

Video Experimental Relacionado

Updated: May 25, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

La expansión del punto de ramificación en una unión de ADN de tres vías totalmente complementaria.

Tara Sabir¹, Anita Toulmin, Long Ma

¹The School of Chemistry, Alan Turing Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK.

Journal of the American Chemical Society

|February 15, 2012

Resumen

Los investigadores estudiaron las uniones de tres vías del ADN (3WJs), encontrando que forman una estructura en forma de Y con una cavidad interna. Este descubrimiento avanza en la comprensión de la estructura del ADN para aplicaciones en nanociencia.

Más Videos Relacionados

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

Videos de Experimentos Relacionados

Last Updated: May 25, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

Área de la Ciencia:

Biología Molecular Biología Molecular
La biofísica es la biofísica.
La nanociencia es la nanociencia.

Sus antecedentes:

Los ácidos nucleicos ramificados, como las uniones de tres vías del ADN (3WJ), son cruciales en procesos biológicos como la replicación, la recombinación y la reparación del ADN.
Estas estructuras también encuentran aplicaciones en la arquitectura de ARN y como bloques de construcción en la nanociencia.

Objetivo del estudio:

Para dilucidar la estructura local y global de las uniones de tres vías de ADN (3WJs) en solución.
Comprender la base estructural de la flexibilidad y la reactividad observadas de 3WJs.

Principales métodos:

Transmisión de energía de resonancia Förster de una sola molécula de alta resolución (FRET).
Espectroscopia con resolución de tiempo.
Modelado molecular y modelado molecular.

Principales resultados:

El ADN 3WJ adopta una conformación piramidal en forma de Y en solución.
Las bases adyacentes al punto de ramificación permanecen sin emparejar, a pesar de la completa complementariedad de Watson-Crick.
Esta separación crea una cavidad a nanoescala en el centro de la unión.

Conclusiones:

La estructura determinada explica las observaciones anteriores con respecto a la flexibilidad y la reactividad de 3WJ.
Estos hallazgos proporcionan una base estructural para el diseño de nuevos receptores supramoleculares y nanosistemas basados en ADN.