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Dos grupos de genes separados codifican la vía biosintética para los meroterpenoides austinol y dehidroaustinol en
Hsien-Chun Lo1, Ruth Entwistle, Chun-Jun Guo
1Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, California 90089, United States.
Journal of the American Chemical Society
|February 15, 2012
Resumen
Dos grupos de genes separados en Aspergillus nidulans son sorprendentemente requeridos para la biosíntesis de meroterpenoides fúngicos. Este estudio aclara la vía biosintética completa, identificando nuevos compuestos e intermedios.
Área de la Ciencia:
- La micología de la micología.
- Química de Productos Naturales Química de Productos Naturales
- Biología Molecular Biología Molecular
Sus antecedentes:
- Los meroterpenoides son productos naturales de hongos derivados de los precursores de los policetidos y terpenoides.
- La comprensión de la biosíntesis de meroterpenoides es clave para la ingeniería de nuevos compuestos y la mejora de la producción.
- Se sabe que el Aspergillus nidulans produce meroterpenoides como el austinol y el dehidroaustinol.
Objetivo del estudio:
- Para elucidar genéticamente la vía biosintética de los meroterpenoides en Aspergillus nidulans.
- Para identificar los grupos de genes específicos responsables de la producción de meroterpenoides.
- Para caracterizar los nuevos productos y productos intermedios de meroterpenoides.
Principales métodos:
- Experimentos de eliminación de genes dirigidos en Aspergillus nidulans.
- Análisis químico de extractos de cepas mutantes.
- Aislamiento e identificación de los compuestos meroterpenoides.
Principales resultados:
- Dos grupos de genes distintos, uno que contiene el gen de la policetida sintasa ausA y otro que contiene el gen de la preniltransferasa ausN, son esenciales para la biosíntesis de meroterpenoides.
- Aislamiento de ácido 3,5-dimetilorselínico y otros diez meroterpenoides, incluidos seis compuestos nuevos.
- Identificación de los intermediarios biosintéticos y los productos de derivación.
Conclusiones:
- La biosíntesis de meroterpenoides en Aspergillus nidulans requiere dos grupos de genes separados.
- Se ha propuesto una vía biosintética completa para estos meroterpenoides fúngicos.
- Los hallazgos proporcionan una base para la ingeniería metabólica de los meroterpenoides.
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