Video Experimental Relacionado
Updated: May 6, 2026
10:51
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
Published on: August 30, 2016
22.2K
Las vesículas COPII se vuelven de gran tamaño por la ubiquitina.
1Department of Cell and Developmental Biology, Center for Genomic Regulation (CRG) and University of Pompeu Fabra, 08002 Barcelona, Spain. vivek.malhotra@crg.eu
Cell
|April 3, 2012
Resumen
Las proteínas grandes como las fibrillas de procollageno se exportan desde el retículo endoplasmático a través de vesículas de gran tamaño. Este proceso es facilitado por la ubiquitinación del componente COPII Sec31p.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- El transporte de proteínas en transporte.
Sus antecedentes:
- Las vesículas convencionales recubiertas con COPII tienen limitaciones en el transporte de moléculas de carga grandes.
- La exportación de proteínas grandes, como las fibrillas de procollageno, desde el retículo endoplasmático presenta un desafío celular significativo.
Objetivo del estudio:
- Investigar el mecanismo por el cual se exporta una gran carga de proteínas desde el retículo endoplasmático.
- Identificar los actores moleculares involucrados en la formación de vesículas de gran tamaño para el transporte de grandes cargas.
Principales métodos:
- El estudio se centró en el papel de los componentes COPII en la formación de vesículas.
- Investigó el impacto de la ubiquitinación de proteínas en el tamaño de las vesículas y la exportación de carga.
Principales resultados:
- Se encontró que la ubiquitinación de Sec31p, un componente COPII, es crucial para la formación de vesículas.
- Esta modificación facilita la creación de vesículas de gran tamaño capaces de acomodar grandes cargas como las fibrillas de procollageno.
Conclusiones:
- La ubiquitinación de Sec31p es un mecanismo regulador clave para la exportación de grandes cargas desde el retículo endoplasmático.
- Este hallazgo proporciona información sobre cómo las células superan las limitaciones de tamaño en las vesículas transportadoras de proteínas.
Videos de Conceptos Relacionados
01:22Enlargement of the Plasma Membrane
1.8K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
1.8K
00:59COP Coated Vesicles
12.8K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
12.8K
01:32Pinching-off of Coated Vesicles
3.2K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K
01:45Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K
01:15Transcytosis of IgG
3.4K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
3.4K
00:55Microvilli
11.3K
Microvilli are tiny finger-like projections found on the surface of certain cells. Their purpose is to increase the surface area of the cell's apical surface, resulting in more effective absorption or secretion of substances.
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
These microvilli are predominantly present in cells lining the small intestine, kidney tubules, and certain cells in the respiratory and reproductive systems. By significantly expanding the surface area of the cell membrane, microvilli enhance the cell's capacity...
11.3K