Video Experimental Relacionado
Updated: May 23, 2026
14:18
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
SARAF desactiva la maquinaria de entrada de calcio operada por el almacén para evitar que se vuelva a llenar el
Raz Palty1, Adi Raveh, Ido Kaminsky
1Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
Cell
|April 3, 2012
Resumen
Los científicos identificaron a SARAF como una proteína que regula la entrada de calcio operado por almacenamiento (SOCE). SARAF actúa como un regulador negativo, controlando los niveles de calcio y protegiendo las células de la sobrecarga.
Área de la Ciencia:
- Biología molecular La biología molecular.
- Fisiología celular La fisiología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La entrada de calcio operada por almacenamiento (SOCE) es crucial para la homeostasis y la función del calcio celular.
- Las proteínas STIM y Orai son componentes clave de los canales CRAC que median SOCE.
Objetivo del estudio:
- Para identificar nuevos reguladores de SOCE.
- Para caracterizar el mecanismo molecular de SARAF en la regulación SOCE.
Principales métodos:
- Expresión heteróloga.
- El silenciamiento mediado por RNAi.
- Mutagénesis dirigida al sitio.
- Electrofisiología y electrofisiología.
- Pruebas bioquímicas de ensayos.
- Técnicas de obtención de imágenes técnicas de imagen.
Principales resultados:
- SARAF fue identificado como un regulador negativo de SOCE.
- SARAF es una proteína residente en el retículo endoplasmático asociada con STIM.
- SARAF actúa como mediador de una lenta inactivación dependiente de Ca2+ de SOCE.
- SARAF influye en las señales citosólicas de Ca2+ y el contenido del almacén intracelular de Ca2+.
Conclusiones:
- SARAF juega un papel crítico en la configuración de las señales de calcio celular.
- SARAF es importante para prevenir la sobrecarga de calcio celular.
- SARAF representa un nuevo objetivo para la modulación de SOCE.
Videos de Conceptos Relacionados
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
01:27Feedback Regulation of Calcium Concentration
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
01:18Antihypertensive Drugs: Action of Calcium Channel Blockers
Calcium ions are essential to contract smooth muscle cells in blood vessels. They enter these cells through voltage-dependent calcium channels, specifically L-type calcium channels in the cell membrane. These L-type calcium channels are integral to the excitation-contraction coupling process in smooth muscle. When a stimulus is received by smooth muscle cells, their membrane depolarizes. This alteration in membrane potential instigates the opening of L-type calcium channels. As a result,...
01:29Relaxation of Skeletal Muscles
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open.
01:21Skeleton and Calcium Homeostasis
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...