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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
La regulación coordinada de elementos genéticos accesorios produce di-nucleótidos cíclicos para la virulencia de V.
Bryan W Davies1, Ryan W Bogard, Travis S Young
1Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA.
Cell
|April 17, 2012
Resumen
El Vibrio 7th pandemic island-1 (VSP-1) regula la patogénesis del cólera a través de un nuevo factor de transcripción de ARN pequeño y VspR. Esta vía produce un di-nucleótido cíclico crucial para la colonización intestinal y la adaptación del huésped.
Área de la Ciencia:
- Microbiología Microbiología.
- La genómica es la genómica.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El papel del Vibrio 7th pandemic island-1 (VSP-1) en la patogénesis del cólera era previamente desconocido.
- Comprender la regulación del factor de virulencia es clave para combatir las infecciones por Vibrio cholerae.
Objetivo del estudio:
- Para aclarar la función de VSP-1 en la patogénesis del cólera.
- Identificar las vías reguladoras que conectan VSP-1 y otros factores de virulencia.
Principales métodos:
- La secuenciación de la inmunoprecipitación de la cromatina (ChIP-seq) para mapear el ToxT regulon.
- Secuenciación de ARN para identificar pequeños reguladores de ARN.
- Análisis de expresión génica y ensayos funcionales en V. cholerae.
Principales resultados:
- Un pequeño ARN de la isla TCP desregula VSP-1 codificado VspR.
- La VspR regula genes que incluyen una nueva di-nucleótido ciclasa (DncV).
- DncV sintetiza una molécula híbrida cíclica AMP-GMP esencial para la colonización y la regulación de la quimiotaxis.
Conclusiones:
- VSP-1 es una isla de patogenicidad en V. cholerae, crucial para la adaptación del huésped.
- Esta vía conecta islas genómicas dispares y revela un nuevo mecanismo regulador.
- La producción de di-nucleótido cíclico mediada por DncV es vital para la virulencia de V. cholerae.
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